高效液相色谱法同时测定熟肉制品中日落黄、 胭脂红和诱惑红

Determination of sunset yellow, carmine and allura red in cooked meat products by high performance liquid chromatography

中文关键词:  熟肉制品  合成色素  高效液相色谱法  测定

英文关键词:cooked meat products  synthetic pigments  high performance liquid chromatography  determination

中文摘要:

      目的 建立对熟肉制品中日落黄、胭脂红和诱惑红三种合成色素同时测定的方法。方法 样品采用无水乙醇-氨水-水(7: 2: 1)提取, 加正己烷去除脂肪, 氮吹后定容, 用高效液相色谱法测定。经Symmetry Shield RP18柱分离, 甲醇-0.02 mol/L乙酸铵溶液梯度洗脱, 检测波长为508 nm。结果 本方法的相对标准偏差(n=6)在0.92%～3.2%之间, 平均回收率在86.4%～99.1%之间, 检出限在0.005～0.008 mg/kg之间。结论 本方法灵敏度高, 分离效果好, 结果稳定可靠, 适用于熟肉制品中日落黄、胭脂红和诱惑红的同时测定。

英文摘要:

      Objective To establish a method of simultaneous determination of sunset yellow, carmine and allura red up to three synthetic pigments in cooked meat products. Methods The samples were extracted by the anhydrous ethanol-ammonia-water solution (7:2:1), the fat was eliminated by hexane, then blown by nitro-gen, analyzed by high performance liquid chromatography. The samples were separated by SymmetryShield RP18 column, by gradient elution using a mobile phase made up of methanol and 0.02 mol/L amm-onium acetate, and detected at 508 nm. Results The relative standard derivations (n=6)were between 0.92%~3.2%, the recoveries were between 86.4%~99.1%, the detection limits were between 0.005～0.008 mg/kg. Conclusion This method is sensitive, efficient, reliable and suitable for the determination of synthetic pigments in cooked meat products.