超高效液相色谱-电喷雾串联四极杆质谱法检测芦笋中20种氨基甲酸酯类农药残留

Detection of 20 carbamate pesticides in asparagus by ultra performance liquid chromatography-electrospray ionization-mass spectrometry-mass spectrometry

中文关键词:  超高效液相色谱-电喷雾串联四极杆质谱法  氨基甲酸酯类  农药  芦笋制品

英文关键词:ultra performance liquid chromatography-electrospray ionization tandem quadrupole mass spectrometry  carbamates  pesticides  asparagus products

中文摘要:

      目的 建立芦笋及其罐头制品中20种氨基甲酸酯类农药残留的定性定量分析方法。方法 采用改进的QuEchERS法提取和净化, 利用超高效液相色谱-电喷雾串联四极杆质谱仪, 在多反应监测正离子扫描模式下对样品进行添加回收率试验。结果 分别对绿芦笋、白芦笋、绿芦笋罐头、白芦笋罐头4种空白基质添加0.005~0.050 mg/kg农药样品进行回收率试验, 回收率为62.44%~85.99%, 定量限均为0.005 mg/kg, RSD均小于9%。结论 该方法简单快速, 灵敏度高, 能够同时满足芦笋及其罐头制品中20种氨基甲酸酯类农药残留的检测要求。

英文摘要:

      Objective To establish an ultra performance liquid chromatography-electrospray ionization- mass spectrometry-mass spectrometry method for detecting of 20 kinds of carbamate pesticides in asparagus and asparagus products. Methods Samples were extracted by modified QuEchERS method, and the mode of multi-reaction monitoring positive ion scanning was applied for analysis by ultra high performance liquid chromatography-electrospray tandem quadrupole mass spectrometer. Results The blank matrixes (green asparagus, white asparagus, canned white asparagus, canned white asparagus) were added 0.005~0.050 mg/kg carbamate pesticides separately and recovery tests were performed. Results demonstrated that the recovery rates were in the range of 62.44%~85.99% and the limits of quantification were 0.005 mg/kg for all the 20 carbamate pesticides tested with the relative standard deviations (RSDs) below 9%. Conclusion The method, not only simple and fast but also high sensitive, can satisfy the international detection requirement for 20 carbamate pesticides residue in asparagus product simultaneously.