超高效液相色谱法检测焙烤食品馅料中的 二氧化硫脲

Determination of thiourea dioxide in fillings of bakery food by ultra performance liquid chromatography

中文关键词:  馅料  二氧化硫脲  超高效液相色谱-二极管阵列检测法

英文关键词:fillings of bakery food  thiourea dioxide  ultra performance liquid chromatography-photo-diode array

中文摘要:

      目的 建立馅料中二氧化硫脲的超高效液相色谱测定方法。方法 用0.05%乙酸水溶液提取, 样品提取液经过离心, 乙腈稀释过滤膜过滤后, 以HILIC色谱柱为分析柱分离, 采用超高效液相色谱-二极管阵列检测器(ultra performance liquid chromatography-photo-diode array, UPLC-PDA)检测, 以乙腈和水为流动相, 梯度洗脱, 检测波长为284 nm。采用外标法定量。结果 二氧化硫脲的线性范围为0.4~20.0 mg/L, 相关系数不小于0.999, 空白样品的加标回收的回收率为80.6%~102.2%, 相对标准偏差为2.6%~5.0%。结论 该方法简便、快速、准确、重现性好, 适用于馅料中二氧化硫脲的检测。

英文摘要:

      Objective To establish a method to detect the content of thiourea dioxide in fillings of bakery food by using ultra performance liquid chromatography (UPLC). Methods The thiourea dioxide was extracted by 0.05% acetic acid, and then centrifuged. The extracts was separated by HILIC column, and detected by the photo-diode array (PDA) at the wavelength of 284 nm. Results The thiourea dioxide concentration and the peak area showed a good linearity from 0.4 to 20 mg/L with the correlation (r) no less than 0.999. The average recoveries of the spiked blank fillings of bakery food were from 80.6% to 102.2% with the relative standard deviations (RSD) in the range of 2.6%~5.0%. Conclusion The method was simple, quick, accurate and robust for the detection of thiourea dioxide in fillings of bakery food.