杯碟法和酶热仪法检测乳及乳制品中β-内酰胺酶类物质的比较

Comparison of cylinder plate method and enzyme thermistor method for detection of β-lactamase activity in milk and dairy products

中文关键词:  β-内酰胺酶  酶热生物传感器  杯碟法  牛乳

英文关键词:β-lactamase  enzyme thermistor biosensor  cylinder plate method  milk

中文摘要:

      本文对β-内酰胺酶的多种国内外检测方法进行了介绍, 并着重比较了杯碟法和酶热仪法的特点, 详细分析了两种方法在检测原理、仪器设备、实验操作、实验周期、结果判定、方法学参数等方面的异同点。杯碟法稳定可靠、灵敏度高, 是目前中国乳及乳制品中β-内酰胺酶检测的推荐方法; 酶热仪法简便快捷、可实现半定量检测, 更适合于大规模样品筛查和现场检测。两种方法的测定结果具有可比性, 可根据检测要求和实验室情况配合使用。

英文摘要:

      An overview of the determination methods for β-lactamase in milk and dairy products was presented, with discussion of the advantages and disadvantages of cylinder plate and enzyme thermistor biosensor method in detail. The advantages of reliability and sensitivity had been made the cylinder plate method a rec-ommended strategy for β-lactamase test in China. Compared to the conventional culture-based assay, enzyme thermistor biosensor provided a rapid, simple and semi-quantification tool for routine use and on-site inspection. Both of the two methods were competent for β-lactamase analysis in milk and dairy products, showing results in a good agreement.