| 赵 芳,洪小柳,万志刚,葛丽雅,黄李华,刘慧玲,薛 峰,蒋 原,吕敬章,张 玲.进出口食品中金黄色葡萄球菌spa基因的分型[J].食品安全质量检测学报,2014,5(11):3462-3466 |
| 进出口食品中金黄色葡萄球菌spa基因的分型 |
| The spa genotyping of Staphylococcus aureus isolated from import and export food |
| 投稿时间：2014-10-11 修订日期：2014-11-19 |
| DOI： |
| 中文关键词: 金黄色葡萄球菌 spa基因 多态性分析 |
| 英文关键词:Staphylococcus aureus spa gene SNP typing |
| 基金项目:国家科技支撑计划(2012BAK17B10)、高技术研究发展计划(863计划)(2012AA101601)、质检公益项目(201510025, 201210127) |
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| Author | Institution |
| ZHAO Fang | Shenzhen Key Laboratory of Detection Technology R D on Food Safety, Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau |
| HONG Xiao-Liu | Shenzhen Key Laboratory of Detection Technology R D on Food Safety, Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau |
| WAN ZHI-Gang | Shenzhen Key Laboratory of Detection Technology R D on Food Safety, Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau |
| GE LI-Ya | Shenzhen Key Laboratory of Detection Technology R D on Food Safety, Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau |
| HUANG LI-Hua | Shenzhen Key Laboratory of Detection Technology R D on Food Safety, Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau |
| LIU Hui-Ling | Shenzhen Key Laboratory of Detection Technology R D on Food Safety, Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau |
| XUE Feng | Testing Center of Animals, Plants and Food, Jiangsu Entry and Exit Inspection and Quarantine Bureau |
| JIANG Yuan | Testing Center of Animals, Plants and Food, Jiangsu Entry and Exit Inspection and Quarantine Bureau |
| LV Jing-Zhang | Shenzhen Key Laboratory of Detection Technology R D on Food Safety, Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau |
| ZHANG Ling | Department of Transfusion Medicine, School of Biotechnology, Southern Medical University |
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| 中文摘要: |
| 目的 对我国进出口食品中分离得到的金黄色葡萄球菌进行spa基因分型。方法 对36株金黄色葡萄球菌的spa基因进行PCR扩增, 并对产物进行测序分析, 测序结果通过数据库进行spa基因分型。结果 共分出16个基因型, 分别为t189、t091、t164、t002、t899、t197、t1044、t034、t156、t7400、t2592、t4209、t127、t437和两株新发现的基因型。其中t189型共有13株, t091型共4株, t164和t002型分别为3株, 其余型分别为1株。结论 食品中分离出的36株金黄色葡萄球菌spa分型以t189居多, t091型次之, 其他14型相对较少。 |
| 英文摘要: |
| Objective To understand of the spa genotyping of Staphylococcus aureus in import and export food. Methods The X region of the spa gene was amplified by PCR. DNA sequences were obtained with an sequencer and the spa types were determined with the database accessible via http://www. ridom.de/spaserver/. Results Thirty-six strains can be divided into 16 types, including t189, t091, t164, t002, t899, t197, t1044, t034, t156, t7400, t2592, t4209, t127, t437 and 2 new types. There are 13 strains t189 type, 4 strains t091 type, 3 strains of t164 type and 3 strains t002 type, residual type 1 strains respectively. Conclusion The main spa typing of 36 strains of Staphylococcus aureus in import and export food is t189 type, followed by t091 type, and the other 14 types less. |
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