超高效液相色谱法快速检测麦卢卡蜂蜜中丙酮醛

Determination of methylglyoxal in Manuka honey by ultra performance liquid chromatography

中文关键词:  麦卢卡蜂蜜  超高效液相色谱法  丙酮醛

英文关键词:Manuka honey  ultra performance liquid chromatography  methylglyoxal

中文摘要:

      目的 利用超高效液相色谱建立一种麦卢卡蜂蜜中丙酮醛的快速检测方法。方法 采用乙酸钠缓冲溶液直接提取蜂蜜中的丙酮醛, 提取液与2,4-二硝基苯肼溶液室温避光衍生20 h后, 经C18色谱柱分离后采用二极管阵列检测器(PDA)检测, 外标法定量。结果 丙酮醛的检出限为10.0 mg/kg, 定量限为25.0 mg/kg, 在1.0~60 mg/L之间线性关系良好, 相关系数为0.9982, 在蜂蜜中添加25.0、500、1000三个水平的回收率在93.9%~97.8 %之间, 相对标准偏差在 1.8%~5.4%之间。结论 该分析方能够满足麦卢卡蜂蜜中丙酮醛的快速测定要求。

英文摘要:

      Objective To establish a fast detection method of methylglyoxal (MGO) in Manuka honey by ultra performance liquid chromatography (UPLC). Methods MGO in honey was extracted by sodium acetate buffer solution, extract solution derivatized 20 h with 2,4-dinitrophenylhydrazine solution, and detected by PDA detector after separated by C18 chromatographic column, quantified by external standard calibration method. Results The detection limit of MGO was 10.0 mg/kg, limit of quantification was 25.0 mg/kg. The method showed a good linear range of detection between 1.0~60 mg/L mg/L (r2=0.9982). The recoveries of MGO at three spiked levels (25.0, 500, and 1000 mg/kg) were between 93.9% ~ 97.8 %, RSD between 1.8% ~ 5.4%. Conclusion The method is suitable for fast analysis of MGO in Manuka honey.