弱酸性电位水对单增李斯特菌致死机制的研究

Lethal injury mechanism of slightly acidic electrolyzed water on

中文关键词:  弱酸性电位水  次氯酸钠  稀盐酸  单增李斯特菌  致死机制

英文关键词:slightly acidic electrolyzed water  sodium hypochlorite  hydrochloric acid  Listeria monocytogenes  sterilization mechanism

中文摘要:

      目的 研究弱酸性电位水(slightly acidic electrolyzed water, SAEW)对单增李斯特菌的致死机制, 并与强碱性杀菌剂次氯酸钠(NaClO)和强酸性杀菌剂稀盐酸(HCl)进行比较。方法 测定三种杀菌剂的杀菌效率及处理后胞内蛋白泄漏量、TTC-脱氢酶相对活性、Na+/K -ATP酶活性和细菌超微结构的变化。结果 30 mg/L SAEW 和NaClO对Na /K -ATP酶和TTC-脱氢酶相对活性的抑制无显著性差异, 但其显著高于稀HCl组。SAEW处理单增李斯特菌1 min后所导致的蛋白质泄漏量(0.35 mg/mL)显著高于NaClO和稀HCl处理组(分别为0.16 mg/mL和0.11 mg/mL)。SAEW、NaClO、稀HCl对单增李斯特菌的细胞超微结构都造成了明显的损伤, 细胞质凝集, 透电子区扩大。其中稀HCl对超微结构的损伤最大, 说明强酸性环境对细胞形态结构有很大的影响。结论 SAEW处理对单增李斯特菌细胞质造成了一定程度的损伤, 抑制了细胞呼吸作用, 改变了细胞膜的通透性, 降低了细胞膜上的Na /K -ATP酶活性, 导致细胞内大分子物质泄漏而最终导致细胞死亡。SAEW杀菌机制的初步研究为未来SAEW的杀菌研究和应用前景奠定基础。

英文摘要:

      Objective To research the lethal injury mechanism of slightly acidic electrolyzed water on Listeria monocytogenes in comparison with traditional disinfectants such as sodium hypochlorite (NaClO) and hydrochloric acid (HCl). Methods The inactivation of L. monocytogenes by SAEW, NaClO, and HCl was determined while the disinfection mechanism was investigated by measuring changes to intracellular protein leakage, TTC-dehydrogenase relative activity, Na+/K -ATPase activity and cellular ultrastructure as indicators. Results 30 mg/L SAEW and NaClO treatments had no significant difference of inhibition on the activity of Na /K -ATPase and relative activity of TTC-dehydrogenase, but they were significantly higher than that by dilute hydrochloric acid treatment. However, the protein leakage from L. monocytogenes caused by SAEW in 1 min (0.35 mg/mL) was significantly higher than that of NaClO and HCl treatment (0.16 mg/mL and 0.11 mg/mL). SAEW, NaClO and dilute HCl caused remarkably damages on the ultrastructure of L. monocytogenes, partly cytoplasm agglutination and expansion of electron transmission. The maximum damages were caused by dilute HCl which illustrated that strong acid has a great impact on cell morphology. Conclusion SAEW partly disrupted the cytoplasm of cell, inhibited cellular respiration, changed the permeability of cell membrane, induced intracellular macromolecules leakage and eventually lead to cell death. This preliminary research which focused on the sterilization mechanism of SAEW had laid the foundation for the future investigations and application of SAEW.