高效液相色谱法测定蜂王浆中6种糠醛类物质含量

Determination of 6 furfural compounds contents in royal jelly by high per-formance liquid chromatography

中文关键词:  高效液相色谱法  蜂王浆  糠醛类物质

英文关键词:high performance liquid chromatography  royal jelly  furfural compounds

中文摘要:

      目的 建立同时测定蜂王浆中5-羟甲基-2-糠醛 (5-HMF)、5-甲基糠醛 (5-MF)、2-糠醛 (F)、2-糠酸 (2-OIC)、3-糠酸 (3-OIC)、5-羟甲基-2-糠酸 (5-HMFacid) 等6种糠醛类物质含量的高效液相色谱分析方法。方法 样品经去离子水提取, 亚铁氰化钾和乙酸锌溶液沉淀蛋白后, 用C18色谱柱分离, 0.5%甲酸水溶液和甲醇梯度洗脱, 在各化合物的最佳吸收波长处进行检测。结果 在测试范围内, 6种糠醛类物质的浓度与相应的峰面积呈线性关系, 相关系数(R2)不小于0.9993。方法定量和定性检出限分别在0.07~0.26 μg/mL和0.02~0.08 μg/mL之间, 加标回收率为86.2%~99.7%, 相对标准偏差小于2.40%。结论 该方法操作简单, 灵敏度高, 适合蜂王浆中6种糠醛类化合物的测定。

英文摘要:

      Objective To establish a reversed-phase high performance liquid chromatography method for determining 5-hydroxymethyl-2-furfural (5-HMF), 5-methyl-2- furaldehyde (5-MF), 2-furaldehyde (F), fu-ran-2-carboxylic acid (2-OIC), furan-3-carboxylic acid (3-OIC) and 5-hydroxymethyl-2-furancarboxylic acid (5-HMF acid) in royal jelly (RJ). Methods Samples were extracted with purity water and proteins in them were removed by zinc acetate and potassium ferrocyanide solutions. Before injection, the solutions were passed through 0.45 μm filters for determination by a liquid chromatography UV detection at different wavelengths. Results Within the test ranges, 6 furfural compounds concentrations and their peak areas showed good linear correlations (R2≥0.9993). The limits of quantitation and detection were 0.07~0.26 μg/mL and 0.02~0.08 μg/mL, respectively. The recoveries ranged from 86.2% to 99.7% and the overall relative standards were lower than 2.40%. Conclusion The method is simple, sensitive and suitable for analyzing 6 furfural compounds in RJ.