吉氏芽胞杆菌来源来氟米特分析与检测

Analysis and testing on leflunomide from Bacillus gibsonii FJAT-10019

中文关键词:  液相四级杆飞行时间质谱法  吉氏芽胞杆菌  胞外代谢物  来氟米特

英文关键词:liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry  Bacillus gibsonii  extracellular metabolites  leflunomide

中文摘要:

      目的 对吉氏芽胞杆菌Bacillus gibsonii FJAT-10019来源的来氟米特成分进行分析与检测。方法 采用高效液相四级杆飞行时间质谱分析吉氏芽胞杆菌Bacillus gibsonii FJAT-10019发酵液中胞外代谢物的成分。运用安捷伦公司的MassHunter软件, 对数据进行分子特征提取(MFE), 并通过Metlin代谢物质谱数据库检索, 获得总体代谢物的信息。结果 在吉氏芽胞杆菌Bacillus gibsonii FJAT-10019发酵液中, 用液相质谱检测到561种代谢物, 通过Metlin谱库搜索得到初步鉴定结果的有15种。其中, 来氟米特是相对含量和匹配率最高的成分。来氟米特的匹配得分达到90.89, 占其发酵液总代谢物相对含量的1.14%。来氟米特的保留时间为6.522分钟, 精确质量数为270.0623。结论 为吉氏芽胞杆菌来源的该免疫调节剂的开发与利用提供理论依据。

英文摘要:

      Objective To analyze and test on leflunomide from Bacillus gibsonii FJAT-10019. Methods Extracellular metabolites of Bacillus gibsonii FJAT-10019 were analyzed by liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry. Molecular Feature Extraction(MFE)and database retrieval wereapplied by Agilent MassHunter software, for the information overall metabolites. Results Totally 561 compounds were detected, and 15 compounds were identified by library searching of Metlindatabase. The compound of leflunomide with the match score 90.89, and relative content is 1.14%. The retention time of leflunomide is about 6.522 min, accurate mass is 270.0623. Conclusion This result provided the theory basis for development and utilization of this immunomodulator from Bacillus gibsonii.