等吸光点紫外分光光度法测定红三叶草中总异黄酮含量的研究

Isoabsorptive point determination of content of red clover isoflavones by ul-traviolet spectrophotometry

中文关键词:  芒柄花黄素  鸡豆黄素  等吸光点  紫外分光光度法

英文关键词:formononetin  biochanin A  isoabsorptive point  ultraviolet spectrophotometry

中文摘要:

      目的 应用等吸光点紫外分光光度法测定红三叶草中总异黄酮含量。方法 本文以芒柄花黄素(formononetin)和鸡豆黄素(biochanin A)为混合标准品, 应用紫外分光光度法在等吸光收点处(253.5nm)测定红三叶草中总异黄酮含量。结果 研究表明等吸光点处的总吸光度与总异黄酮含量相关, 而与二者的相对比例无关。测定的线性范围为2～8 μg/mL, 加样回收率为97.3%, 相对标准偏差为1.08%。结论 与已有的单标准品测试方法相比, 以芒柄花黄素(formononetin)和鸡豆黄素(biochanin A)为混合标准品的实验方法更加稳定、准确, 并且综合等吸光点紫外分光光度法快速、重复性好的优点, 使得此方法适用于红三叶草中异黄酮含量检测, 可为一般的红三叶草异黄酮的提取纯化研究和生产厂家提供参考。

英文摘要:

      Objective Isoabsorptive point ultraviolet spectrophotometry was used to determine the content of isoflavones in red clover. Methods The content of isoflavones in red clover was determined by ultraviolet spectrophotometry at isoabsorptive point (235.5 nm) and compared with the formononetin and biochanin A as mixture standard. Results The research showed that the absorbance at isoabsorptive point was related with the content of isoflavones but no relation with the respective percentage. The linear range of determination was 2~8 μg/mL, the average recovery was 97.3%, and the RSD was 1.08%. Conclusion Compared to conventional method using single standard, the detection with the mixture standard of formononetin and biochanin A is more steadily and accurately, with a rapid and good repeatability of isoabsorptive point. This method is suitable for the determination of content of red clover isoflavones, and can provide reference for extraction and purification of red clover isoflavones and manufacture factory.