高效液相色谱法测定调味面制品中 富马酸二甲酯

Determination of dimethyl fumarate in seasoned flour products by high performance liquid chromatography

中文关键词:  富马酸二甲酯  防霉剂  调味面制品  高效液相色谱

英文关键词:dimethyl fumarate  antiseptic  seasoned flour products  high performance liquid chromatography

中文摘要:

      目的 建立调味面制品中富马酸二甲酯快速、灵敏的高效液相色谱测定方法。方法 色谱柱为ODS-C18 4.6 mm×250 mm, 流动相为甲醇+(0.02 mol/L)乙酸铵(55:45, v:v)。样品粉碎用氨水甲醇溶液超声提取后, 用盐酸调节pH为6, 经0.45 μm滤膜过滤, 进样测定, 保留时间定性, 峰面积定量。结果 标准曲线线性良好, 方法线性范围0.25~10 mg/L, 线性相关系数为0.9995, 检出限为0.03 mg/kg, 定量限为0.1 mg/kg, 加标回收率在94.0%~102.5%范围内, 相对标准偏差在3.6%~5.2%之间。结论 该方法操作简便、具有灵敏高、准确性好、无杂质干扰等优点, 可用于调味面制品中富马酸二甲酯的测定。

英文摘要:

      Objective To establish a rapid and sensitive high performance liquid chromatography method for determination of demethyl fumarate in seasoned flour products. Methods Chromatographic column was ODS-C18 4.6 mm×250 mm. The mobile phase was methanol 0.02 mol/L ammonium acetate (55:45, v:v). Sample was crushed and extracted with ammonia water methanol solution; pH value was adjusted to 6 with hydrochloric acid; 0.45 μm membrane was used for filtration after ultrasonic extraction. Then, the sample was injected into high performance liquid chromatography, the component was qualitatively determined by retention time and quantitatively determined by peak area. Results Under the optimal conditions, the method showed a good linearity in the range of 0.25~10.0 mg/L, and the correlation coefficient was 0.9995. The limit of detection was 0.03 mg/kg, and the limit of quantification was 0.1 mg/kg. The recovery percentages varied from 94.0% to 102.5% and relative standard deviations were in the range 3.6%~5.2%. Conclusion The method was proved to be satisfactory in precision, accuracy and sensitivity, and without the distraction of impurities. It can be used to detect demethyl fumarate in seasoned flour products.