一株啶虫脒降解菌AC04的鼠类急性毒性评价

Acute toxicity evaluation of a strain of bacteria AC04 in rodent models

中文关键词:  微生物降解  啶虫脒  急性毒性  安全性评价

英文关键词:microbial degradation  acetamiprid  acute toxicity  safety evaluation

中文摘要:

      目的 对从长期种植枸杞的土壤中分离筛选出的一株可高效降解农药啶虫脒的细菌AC04进行急性毒性的安全性评价。方法 将提取的AC04菌液对小鼠进行经口灌胃急性毒性实验; 将表面喷洒菌悬液的枸杞对大鼠进行30 d喂养实验。结果 经口急性毒性实验中实验小鼠行为、进食均正常, 未出现中毒及死亡情况。观察期结束后, 解剖动物主要脏器、器官, 均未见明显异常改变。大鼠30d喂养实验中, 实验动物的体征外观、行为、体重、血象等各项指标均正常, 无明显中毒症状或不良反应。结论 该菌株及其代谢产物对试验鼠未见明显毒性。

英文摘要:

      Objective To evaluate a new screened strain of bacteria AC04 found in the soil of long-term planting of Chinese wolfberry which can efficiently degrade the pesticide acetamiprid using rodent acute toxicity models. Methods The extracted fluid of bacteria was given to mice for acute toxicity experiment by ig. The rats were fed with wolfberry sprayed by the bacterial suspension for 30 d. Results The acute oral toxicity tests showed the tested mice behaving and eating normal, without poisoning and death. At the end of the observation, the mice were dissected and the major organs showed no abnormalities. In the experiments of rats fed for 30 d, the appearance of experimental rats, as well as the behavior, body weight, and blood indicators showed normal, without obvious symptoms or adverse reactions. Conclusion The research showed that this strain of bacteria was harmless to the experimental animals.