无需衍生化样品处理快速测定食品中 黄曲霉毒素B1

Rapid determination of aflatoxin B1 in foods without derivation

中文关键词:  黄曲霉毒素B1  超高效液相色谱法  免疫亲和柱  无衍生

英文关键词:aflatoxin B1  ultra performance liquid chromatography  immuno-affinity column  without derivation

中文摘要:

      目的 建立无需衍生化法-超高效液相色谱直接测定食品中的黄曲霉毒素B1的快速检测方法。 方法 样品经甲醇-水(7:3, v:v)提取, 免疫亲和柱净化, C18色谱柱分离后, 采用大体积流通池荧光检测器检测。结果 黄曲霉毒素B1在0.10~5.00 ng/mL范围内与峰面积呈良好线性关系, R2>0.999, 在饼干、粉丝、点心、沙琪玛、蛋糕、辣椒酱6种基质中, 黄曲霉毒素B1在0.50~2.00 μg/kg范围内加标, 平均回收率在71.7%~111.2%之间, 相对标准偏差(RSDs)为3.8%~11.5%, 检出限(LOD)为0.05 μg/kg。结论 本方法灵敏、快速、无衍生、结果准确, 能够满足食品中黄曲霉毒素B1残留的检测需求。

英文摘要:

      Objective To establish a rapid determination of aflatoxin B1 in food by ultra performance liquid chromatography (UPLC) without derivation method. Methods Samples were extracted with methanol water (7:3, v:v), cleaned up by immuno-affinity column, separated by C18 column, and determined by fluorescence detector with large volume flow cell. Results High correlation coefficient (r2>0.999) was obtained in the linear range of 0.10~5.00 ng/mL. The average recovery rates were 71.7%~111.2% at the spiked level of 0.50~2.00 μg/kg and relative standard deviation(RSD) was 3.8%~11.5% in biscuit, vermicelli, refreshment, sacima, cakes, and chili sauce. The limit of quantitation (LOD) was 0.05 μg/kg. Conclusion This method is sensitive, rapid, non-derived, and accurate, and it can meet the requirements for determination of aflatoxin B1 in foods.