高效液相色谱-串联质谱法测定饮料、牛奶和奶粉中双酚A和15种邻苯二甲酸酯

Determination of bisphenol A (BPA) and 15 phthalate esters (PAEs) by high performance liquid chromatography-tandem mass spectrometry in beverage, milk and milk powder

中文关键词:  邻苯二甲酸酯  双酚A  高效液相色谱串联质谱

英文关键词:phthalate esters  bisphenol A  high performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立了饮料、牛奶和奶粉中双酚A及15种邻苯二甲酸酯的高效液相色谱?串联质谱分析方法。方法 样品经甲醇提取, C18色谱柱分离后, 采用串联四极杆质谱电喷雾电离源正负离子切换多反应离子监测模式检测。结果 双酚A及15种邻苯二甲酸酯在各自的线性范围内线性关系良好, 相关系数均不低于0.9900, 定量下限为10 μg/kg, 低、中、高3个加标水平下平均回收率为61.1%~129.5%, 相对标准偏差为1.13%~22.12%。结论 该方法具有前处理简单、灵敏度高的优点, 适用于饮料、牛奶及奶粉中双酚A及15种邻苯二甲酸酯残留的确证和定量检测。

英文摘要:

      Objective A method for the simultaneous determination of bisphenol A (BPA) and fifteen phthalate esters (PAEs) by high performance liquid chromatography-tandem mass spectrometry was established. Methods The samples were extracted by methanol. The target compounds were separated by C18 column and detected by MS/MS system with eletrospray ionization (ESI) positive and negative ion mode under multiple reaction monitoring (MRM) mode. Results High correlation coefficients (r≥0.9900) of sixteen compounds were obtained within their respective linear ranges. The LOQ of BPA and 15 PAEs was 10 μg/kg. The recov-eries ranged from 61.1%~129.5%, with the relative standard deviations of 1.13%~22.12%. Conclusion This method is suitable for the identification and quantification of PAEs and BPA in beverage, milk and milk powder with advantages of simple pretreatment, good purification and high sensitivity.