沙棘果渣总黄酮提取工艺响应面法优化与 抗氧化活性研究

Optimizaton of extraction of total flavones from residue of Hippophae rhamnoides L. using response surface methodology and their antioxidant activity

中文关键词:  沙棘  黄酮  提取  响应面  抗氧化

英文关键词:Hippophae rhamnoides L.  flavonoids  extraction  response surface methodology  antioxidant activity

中文摘要:

      目的 优选沙棘果渣总黄酮提取的最佳工艺, 并对其抗氧化活性进行评价。方法 选取提取时间、温度、料液比、乙醇体积分数为影响因素, 在单因素实验结果基础上确定各因素的分析水平, 利用响应面中心组合法设计实验方案。并通过体外抗氧化能力指数(ORAC)法研究沙棘果渣总黄酮的抗氧化能力。结果 建立了沙棘果渣总黄酮提取的数学模型, 最佳提取工艺条件为乙醇体积分数70%、提取温度 80℃、提取时间100 min、液料比25:1, 沙棘果渣总黄酮具有较强抗氧化活性, 与浓度成正相关。结论 本研究结果可为沙棘果渣总黄酮工业化提取和应用提供理论指导。

英文摘要:

      Objective To explore the optimal extraction conditions of total flavonoids from residue of Hippophae rhamnoides L. and evaluate the antioxidant activity. Methods Based on the single-factor tests, the conditions of total flavonoids from residue of Seabuckthorn were optimized by response surface metho-dology in order to increase the extraction yield of total flavonoids. Meanwhile, the antioxidant activity of total flavonoids was assessed by oxygen radical absorbent capacity (ORAC). Results The optimal extrac-tion conditions of total flavonoids were ethanol concentration 70%, material-liquid ratio 1:25, extraction time 100 min and extraction temperature 80℃. Under the optimal conditions, the maximum extraction yield of total flavonoids was 2.73%. Extracted total flavonoids had a strong antioxidant activity in a dose-dependent manner. Conclusion These investigations will be reference a certain theoretical instruction and application value for the extraction of flavonoids in Seabuckthorn.