免疫标记分析技术及其在粮食霉菌毒素 检测中的应用研究进展

A review on applications of labeling immunoassay techniques for mycotoxin detection in grain

中文关键词:  霉菌毒素  粮食  免疫标记技术

英文关键词:mycotoxin  grain  immunolabelling technique

中文摘要:

      霉菌毒素是由丝状真菌产生的次生代谢产物, 严重污染粮食如大米、玉米、小麦、大麦等, 给人类健康带来威胁。为了保护消费者健康和减少经济损失, 监管和控制粮食中的霉菌毒素成为生产者、管理部门和研究人员的主要目标。为此大量快速、适用及精确的检测方法被研究开发并应用, 以特异性抗体为基础的免疫分析技术具有广阔的开发应用前景。本文就免疫标记分析技术以及在粮食霉菌毒素检测中的应用进行综述, 包括粮食的霉菌毒素的种类和对人类的危害; 免疫标记分析技术的分类; 放射免疫分析、酶标记免疫分析、免疫荧光标记技术、时间分辨荧光免疫分析技术、免疫胶体金标记技术等在粮食霉菌毒素检测中的应用

英文摘要:

      Mycotoxins are natural contaminants produced by a range of fungal species. They usually presents in grain such as rice, corn, wheat, and barley, which posses a threat to the health of humans. To protect consumer health and reduce economic losses, surveillance and control of mycotoxins in grain has become a major objective for producers, regulatory authorities and researchers worldwide. In this paper, labeling immu-noassay techniques, such as radio immunoassay, enzyme immunoassay, fluorescence immunoassay, time resolved fluoroimmunoassay and colloidal gold immunoassay, and its applications in mycotoxin detection of grain were discussed.