ELISA法测定牛奶中链霉素不确定度的评定

Evaluation of the uncertainty in measuring streptomycin in milk by ELISA

中文关键词:  链霉素  酶联免疫吸附法  牛奶  不确定度

英文关键词:streptomycin  ELISA  liquid milk  uncertainty

中文摘要:

      目的 对酶联免疫吸附法(ELISA)测定牛奶中链霉素残留量的测量不确定度进行评定。方法 分析了该方法的不确定度来源, 建立数学模型, 并进行分析后找到主要影响因素。结果 当牛奶中链霉素含量为7.7 ng/mL时, 其扩展不确定度为1.52 ng/mL (k =2)。其中, 测量样品重复性、反应温度及回收率影响较大。结论 采用酶联免疫吸附法(ELISA)测定牛奶中链霉素含量应保证实验具有良好的重复性, 严格控制实验反应条件, 并且保证样品回收率符合要求。

英文摘要:

      Objective The uncertainty in detecting streptomycin in milk by ELISA was evaluated. Methods The source of uncertainty of the whole determination procedure was analyzed, and the mathematical model was developed. Results The main influencing factors and the combined uncertainty were obtained. The expanded uncertainty was 1.52 ng/mL (k=2) with 7.7 ng/mL of the content of streptomycin. The major sources of uncertainty of measurement were the measuring repeatability and the reaction temperature, and the sample recovery rate. Conclusion It is necessary to control repeatability of measurement and experimental reaction conditions to make sure the testing results are accurate by ELISA. It also should en-sure that the sample recovery rate could meet the requirements.