有机磷农药多残留检测化学发光标记物的 合成及鉴定

Synthesis and identification of chemiluminescent markers used in the multi-residue determination of organophosphorus pesticides

中文关键词:  O,O-二乙基硫代磷酰氯  鲁米诺  化学发光标记物  鉴定

英文关键词:O,O-diethylthiophosphoryl chloride  luminol  chemiluminescent markers  identification

中文摘要:

      目的 以O,O-二乙基硫代磷酰氯和鲁米诺为原料, 合成一种新型的化学发光标记物, 用于后续的二乙氧基类有机磷农药多残留化学发光免疫法检测。方法 取鲁米诺和O,O-二乙基硫代磷酰氯溶于二氯甲烷中, 以三乙胺作为缚酸剂, 冰浴下反应8 h后分出有机层, 产物经柱层析纯化, 即得到化学发光标记物。结果 产物经紫外、红外鉴定, 实验结果表明反应产物即为所需的化学发光标记物, 产物在BPCL上进行发光特性测定, 表现出较强的发光能力, 最大发光强度达到9000 mV。结论 本方法合成的化学发光标记物具有较强的发光能力, 能用于后续的二乙氧基类有机磷农药多残留的化学发光免疫检测。

英文摘要:

      Objective Chemiluminescent markers were synthesized using O,O-diethylthiophosphoryl chloride and luminol for the multi-residue determination of organophosphorus pesticides. Methods Luminol and O, O-diethyl phosphoryl chloride were added to dichloromethane under ice-cooling with triethylamine as acid binding agent. After 8 h, the organic layer was separated and purified by column chromatography. Results The product was the chemiluminescent markers after indentation using UV and IR. It had a strong luminescent ability on the BPCL, and the maximum luminous intensity reached 9000 mV. Conclusion The chemiluminescent markers synthesized by this method had a strong luminescence capability, and it could be used in the multi-residue determination of organophosphorus pesticides.