| 刘金华,刘韬,孟日增,聂丹丹,刘阳,薛力刚.利用光纤倏逝波生物传感器检测食品中 大肠杆菌O157:H7[J].食品安全质量检测学报,2014,5(4):1142-1146 |
| 利用光纤倏逝波生物传感器检测食品中 大肠杆菌O157:H7 |
| Detecting Escherichia coli O157:H7 with the fiber-optic evanescent wave biosensor in foods |
| 投稿时间：2014-03-03 修订日期：2014-04-19 |
| DOI： |
| 中文关键词: 光纤倏逝波生物传感器 检测 大肠杆菌O157:H7 |
| 英文关键词:fiber-optic evanescent wave biosensors detection Escherichia coli O157:H7 |
| 基金项目:国家质检总局科技计划项目(2011IK206, 2012IK163) |
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| 中文摘要: |
| 目的 建立一种应用光纤倏逝波生物传感器快速检测食品中大肠杆菌O157:H7的方法。方法 对光纤用大肠杆菌O157:H7抗体包被制备检测探针, 用纳米量子点对抗体进行偶联制备检测抗体, 并确定其检测的灵敏度和特异性, 同时通过对人工污染样品的检测确认该方法检测实际样本的可行性。结果 建立的光纤倏逝波生物传感器检测大肠杆菌O157:H7的灵敏度达到50 CFU/mL, 并具有较强的特异性。结论 利用光纤倏逝波生物传感器检测食品中污染的大肠杆菌O157:H7方法快速、准确, 具有较强应用价值。 |
| 英文摘要: |
| Objective To develop a rapid detection method of E.coli O157:H7 using fiber-optic evanescent wave biosensors. Methods The optical fiber with E. coli O157:H7 antibody coated preparation detection probe and the detection sensitivity and specificity were confirmed. At the same time through the detection of artificially contaminated samples confirm the feasibility of the method for detecting the actual sample. Results The detection sensitivity of E. coli O157:H7 was 50 CFU/mL.This method also had a high specificity. Conclusion The fiber-optic evanescent wave biosensor can provide a new method for rapid screening of food samples that polluted by E. coli O157:H7. |
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