4种黄曲霉毒素B1酶联免疫试剂盒 与液相法检测结果比较

A comparative study on 4 kinds of aflatoxin B1 assay kits and liquid chromatography

中文关键词:  黄曲霉毒素  酶联免疫法  液相色谱法

英文关键词:aflatoxin  enzyme-linked immunosorbent assay  liquid chromatography

中文摘要:

      目的 综合评价4 种不同厂家的黄曲霉毒素B1试剂盒。方法 选择几种食品基质(婴幼儿米粉、花生酱、酱油), 分别用R-Biopharm, Romer, Helica和华安麦科的黄曲霉毒素B1试剂盒检测, 将测定结果与液相色谱法测得结果进行比对。结果 通过与液相色谱法测得结果比较, 华安麦科试剂盒只适合检测婴幼儿米粉样品。Helica和R-Biopharm试剂盒能检测多种基质, 而Helica试剂盒检测结果与液相相符度低, R-Biopharm试剂盒检测米粉时, 本底较高。Romer试剂盒适用于花生酱和酱油样品。结论 为了得到最佳的检测结果, 针对不同的样品基质, 应选择合适的试剂盒。当用酶联法检测出阳性样品时, 需用液相色谱法确认。

英文摘要:

      Objective To compare and evaluate 4 kinds of aflatoxin B1 assay kits and liquid chromatography. Methods Three sample matrixes (infant formula rice flour, peanut butter and soy bean sauce) were detected using RIDASCREEN? aflatoxin B1 assay, AgraQuant? aflatoxin B1 assay, Helica low matrix aflatoxin B1 assay and Huaan magnech aflatoxin B1 assay, and then compared the analysis results with those by liquid chromatography. Results By contrast, Huaan magnech aflatoxin B1 assay applied to only the infant formula rice flour. HELICA low matrix aflatoxin B1 assay and RIDASCREEN? aflatoxin B1 assay could detect more sample matrixes, but the analysis results of Helica low matrix aflatoxin B1 assay were inconsistent with those by liquid chromatography, the background of RIDASCREEN? aflatoxin B1 assay was higher. AgraQuant? aflatoxin B1 assay were applied to peanut butter and soybean sauce. Conclusion In view of the different of the sample matrix, the suitable assay kit needed to be chosen to get the best analysis results. When the results of ELISA were positive, the HPLC method was needed for further validation.