麻痹性贝类毒素常规检测分析方法比较与 研究进展

Advances in conventional methods of assay of paralytic shellfish poisoning toxins

中文关键词:  麻痹性贝类毒素  小白鼠生物法  酶联免疫分析  色谱联用

英文关键词:paralytic shellfish poisoning toxins  mouse bioassay  ELISA  chromatography

中文摘要:

      麻痹性贝类毒素作为贝类产品中一种毒性最强、分布广泛的毒素, 不仅严重威胁人们的身体健康, 而且会造成相当大的经济损失。因此其监测检测方法的研究与改进一直是人们的研究热点。本文分析评述了麻痹性贝类毒素的三种常规检测分析方法的优缺点以及最新研究进展, 并探讨了小白鼠生物法、免疫测定法和色谱联用技术作为主要的检测方法由于原理不同, 结合不同的研究需求其应用的领域。其中, 小白鼠生物测定法虽然概括毒性有效, 但是其灵敏度低、误差大、并且需要大量活体动物而逐渐被色谱技术和免疫测定法所取代, 此外, 神经细胞分析法、毛细管电泳技术和表面等离子体共振传感器技术等方法也逐渐得到应用。不管怎样, 这些方法由于需要专业人员、成本高等问题仍需进一步完善。

英文摘要:

      Paralytic shellfish poisoning toxins in shellfish as potent toxins are widely distributed, not only being a serious threat to people’s health, but also causing considerable economic losses. Therefore researches and improving of their surveillance and testing methods have been one of the research focus. The advantages and disadvantages of the latest research progress of three conventional analytical methods to detect the most common marine toxins are reviewed in the paper. Mouse bioassay, immunoassay and chromatography technology as the primary due to different principles of detection, combining different research areas need of their applications. Although the mouse bioassay generalize toxicity is valid, its low sensitivity and requiring a lot of live animals is gradually being replaced by chromatographic immunoassay technology. In addition, nerve cell analysis, capillary electrophoresis and surface plasmon resonance sensor technology and other methods have gradually been applied. Anyway, because of the requiring professionals, cost issues still need to be improved.