萱藻中多酚类抗过敏活性成分纯化方法的研究

Purification of polyphenols with anti-allergic activity from Scytosiphon lomentsrius

中文关键词:  萱藻  抗过敏  多酚  纯化

英文关键词:Scytosiphon lomentsrius  anti-allergic  polyphenols  purification

中文摘要:

      目的 研究不同的大孔树脂对萱藻多酚的纯化效果, 建立其纯化方法。方法 以透明质酸酶抑制活性为抗过敏活性的评价指标, 通过分析7种不同大孔树脂对萱藻多酚的静态和动态吸附曲线, 建立并优化萱藻多酚的纯化工艺。结果 XDA-1型大孔树脂对萱藻多酚有良好的吸附性能和解吸效果, 优化pH及解吸剂浓度后, 通过一步大孔树脂纯化能够得到纯度达45.27%的萱藻多酚, 其透明质酸酶抑制活性比纯化前提高了1.94倍。结论 建立了萱藻多酚的大孔树脂一步法纯化方法。

英文摘要:

      Objective To select appropriate macroporous resin for preliminary purification of polyphenols from Scytosiphon lomentsrius. Methods Seven different macroporous resins were evaluated with static and dynamic adsorption curves, and pH of sample, concentration of resolving agent were assessed to optimize the purification process. Results XDA-1 macroporous resins have good adsorption and desorption with polyphenols. With the optimized process such as pH and resolving agents, polyphenols in Scytosiphon lo-mentsrius can be got with a purity of 45.27%. Compared with the crude extracts of Scytosiphon lomentsrius, the specific inhibition of hyaluronidase activity improved 1.94 times. Conclusion One step method for purification of polyphenols from Scytosiphon lomentsrius was established.