| 张新,陆晶晶,王丹,周爽,赵云峰,吴永宁.玉米赤霉烯酮全抗原的合成及鉴定[J].食品安全质量检测学报,2014,5(3):791-795 |
| 玉米赤霉烯酮全抗原的合成及鉴定 |
| Preparation and characterization of artificial antigen of zearalenone |
| 投稿时间：2014-01-23 修订日期：2014-03-12 |
| DOI： |
| 中文关键词: 玉米赤霉烯酮 全抗原 基质辅助激光解吸电离-飞行时间质谱仪 |
| 英文关键词:zearalenone complete antigen MALDI-TOF |
| 基金项目:国家自然科学基金项目 (81102133) |
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| Author | Institution |
| ZHANG Xin | College of Food,Wuhan Polytechnic University;Key Laboratory of Food Safety Risk Assessment, Ministry of Health, National Center for Risk Assessment of Food safety |
| LU Jing-Jing | Key Laboratory of Food Safety Risk Assessment, Ministry of Health, National Center for Risk Assessment of Food safety |
| WANG Dan | Key Laboratory of Food Safety Risk Assessment, Ministry of Health, National Center for Risk Assessment of Food safety |
| ZHOU Shuang | Key Laboratory of Food Safety Risk Assessment, Ministry of Health, National Center for Risk Assessment of Food safety |
| ZHAO Yun-Feng | Key Laboratory of Food Safety Risk Assessment, Ministry of Health, National Center for Risk Assessment of Food safety |
| WU Yongning | Key Laboratory of Food Safety Risk Assessment, Ministry of Health, National Center for Risk Assessment of Food safety |
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| 中文摘要: |
| 目的 合成玉米赤霉烯酮(ZEN)的全抗原。方法 将烯醇化的ZEN与阳离子化的载体蛋白(BSA)偶联, 制备高特异性的全抗原, 用高性能基质辅助激光解吸电离-飞行时间质谱仪MALDI-TOF检测偶联物及载体蛋白的分子量, 用商品化的ZEN免疫试剂盒对偶联物进行棋盘滴定验证。结果 全抗原及载体蛋白BSA的分子量分别为69693.5 Da、66297.7 Da, 平均每个BSA连上的ZEN分子个数为10.68 个; 平均每个OVA上连上的ZEN分子个数为5.32 个; 偶联物与免疫试剂盒中ZEN的抗体呈阳性反应。结论 合成的ZEN全抗原为ZEN抗体的制备及免疫学方法的建立奠定了基础。 |
| 英文摘要: |
| Objective To synthesize the complete antigen of zearalenone (ZEN). Method ZEN was coupled with cationic bovine serum albumin (cBSA) via a Mannich reaction. Molecular weights of the complete antigen and carrier protein were measured by MALDI-TOF, respectively. And the performance of complete antigen was further validated by chequerboard titration using a commercial ZEN immunoassay kit. Results The molecular weights of the conjugate and BSA were 69693.5 Da, 66297.7 Da, respectively. There were 10.68 ZEN molecules attached to each BSA molecule, and 5.32 ZEN molecules attached to each OVA molecule. A positive result of the immunoreaction between the synthesized complete antigen and the anti-ZEN antibody from immunoassay kit was observed. Conclusion The preparation of complete antigen of ZEN contributes to the anti-ZEN antibody production and immunoassay development. |
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