高效液相色谱-串联质谱法同时测定 大豆中26种农药的残留量

Simultaneous determination of 26 pesticides in soybeans by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  大豆  农药残留  高效液相色谱-串联质谱

英文关键词:soybean  pesticide residues  high performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立高效液相色谱-串联质谱测定大豆中26种农药的多残留分析方法。方法 样品经乙腈提取, 乙腈饱和正己烷脱脂后, 经C18固相萃取柱净化, 采用高效液相色谱-串联质谱进行测定。结果 26种农药均在20 min分钟内流出, 相关系数r在0.99以上, 回收率均在65.28%~114.67%之间, 重现性良好, 相对标准偏差在10%以内。结论 本方法可一次性检出大豆中26种农药残留, 且简便、准确, 适合大批量样品的测定。

英文摘要:

      Objective A high performance liquid chromatography-tandem mass spectrometric method was developed for the simultaneous determination of 26 pesticides in soybeans. Methods Samples were extracted with acetonitrile, defatted with hexane after acetonitrile saturation, and cleaned up with an SPE-C18 cartridge. Finally, the analyte was determined by high performance liquid chromatography-tandem mass spectrometry. Results All the 26 kinds of pesticides were drained out within 20 minutes. The correlation coefficient r was above 0.99. The recoveries were between 65.28%~114.67%, and the relative standard deviation was below 10%. Conclusion This method is suitable for determining 26 kinds of pesticide in soybean, and this method is rapid, accurate and it can be used for the determination of 26 pesticide residues in soybean.