诃子果实活性成分提取及抗氧化活性研究

Study on extraction and antioxidant activities of bioactive substances from Terminalia chebula Retz. fruit

中文关键词:  诃子果实  提取分离  乙酸乙酯萃取物  抗氧化活性

英文关键词:Terminalia chebula Retz. fruits  separation and purification  ethyl acetate part  antioxidant activity  

中文摘要:

      目的 研究诃子果实不同萃取物及乙酸乙酯相各分离部分的抗氧化活性差异。方法 用超声波法提取诃子果实的活性成分, 依次用乙酸乙酯和正丁醇萃取, 比较不同萃取物的得率、总酚含量并采用DPPH法和ABTS法测定其抗氧化活性, 抗坏血酸(VC)作为阳性对照。结果 乙酸乙酯萃取物得率、总酚含量及抗氧化活性均显著高于其余萃取物(P＜0.05)。将乙酸乙酯萃取物通过Toyoperal 40-C柱进一步分离得到8个部分(Fragment 1-Fragment 8), 其中Fragment 8和Fragment 2的抗氧化活性均高于VC, 且Fragment 2的得率为Fragment 8的3.33倍。结论 诃子果实不同萃取物抗氧化活性不同, 其中乙酸乙酯萃取物的抗氧化活性较高。

英文摘要:

      Objective To investigate the antioxidant activities of different phases from Terminalia chebula Retz. fruit and the different fractions of ethyl acetate phase. Methods Bioactive substances were extracted from the fruits of Terminalia chebula Retz. by ultrasound method, and then the extract was partitioned with ethyl acetate and n-butanol successively. The extract yield, contents of total phenol and antioxidant activity which were evaluated by scavenging capability of ABTS and DPPH free radicals of different fractions were compared, and the antioxidant activity of ascorbic acid (VC) was as a positive control. Results The extract yield, contents of total phenol and antioxidant activity of ethyl acetate fraction significantly higher than other fractions (P＜0.05). The ethyl acetate part was further fractionated into 8 fractions (Fragment 1–Fragment 8) by Toyoperal 40-C chromatography. The antioxidant activities of Fragment 8 and Fragment 2 were higher than those of VC and the yield of Fragment 2 is 3.33 times higher than those of Fragment 8. Conclusion The antioxidant activities of different parts from Terminalia chebula Retz. fruit were different, and antioxidant activities of ethyl acetate part was the best.