不同方法提取姜油及其化学成分的气相色谱-质谱法比较分析

Gas chromatography-mass spectrometry analysis of ginger oil with different extraction methods

中文关键词:  超声复合酶  超声  姜油  气相色谱-质谱法

英文关键词:ultrasonic assisted multi-enzyme  ultrasonic  ginger oil  gas chromatography-mass spectrometry

中文摘要:

      目的　研究不同方法提取姜油成分的不同。方法　以生姜粉为原料，分别采用超声复合酶法和超声法从生姜中提取得到姜油，运用GC-MS分析鉴定研究不同方法下姜油化学成分及相对含量。结果　两种方法提取姜油的得率分别为6.72%?0.02和6.60%?0.04；超声复合酶法提取得到的姜油中共鉴定出53种成分，超声法提取得到的姜油中共鉴定出44种成分，两种方法提取的姜油在主要成分上差别不大，前期和中期主要为萜类及其氧化物，后期主要是姜油树脂成分，但超声复合酶法提取所得姜油成分含量最高的为姜酚25.36%，其次是姜烯18.12%，超声法提取所得姜油成分含量最高的为姜烯24.41%，其次是姜酚20.14%。结论　不同方法提取的姜油在挥发性成分和非挥发性成分含量上存在差异，这为姜油在实际生产中的应用提供参考。

英文摘要:

      Objective To study the components of ginger oil with two different extraction methods. Methods Ginger oil was extracted by ultrasonic assisted multi-enzyme hydrolysis method and ultrasonic–assisted extraction method from ginger powder. And the components of ginger oil were analyzed by gas chromatography-mass spectrometry (GC-MS). Results The average yield of ginger oil with two methods were (6.72?0.02)% and (6.60?0.04)% respectively. Fifty three kinds of components were identified by ultrasonic assisted multi-enzyme hydrolysis method, and the content of gingerol was 25.36%, zigiberene was 18.12%. Forty four kinds of components were identified by ultrasonic-assisted extraction method, and the content of zigiberene was 24.41%, gingerol was 20.14%. There was difference in the components of ginger oil between the two methods. Prophase and metaphase was mainly terpenoids and their oxides, the latter was mainly the zingiber oleoresin. Conclusion　There were differences in the content of volatile and non-volatile components of ginger oil extracted by different methods. This provides the reference for the application of ginger oil in pratical production.