高效液相色谱法检测哌虫啶在稻田水、土壤、 稻米和小麦粉中的残留

Study on residue determination of Paichongding in paddyfield water, soil, rice and wheatby HPLC

中文关键词:  哌虫啶  高效液相色谱法  残留分析

英文关键词:paichongding (IPP)  high performance liquid chromatography  residue analysis

中文摘要:

      目的 建立用高效液相色谱分析稻田水、土壤、稻米和小麦粉中哌虫啶残留的方法。方法 稻田水用二氯甲烷提取, 土壤、稻米和小麦粉用乙腈提取, 采用高效液相色谱紫外检测器进行检测。结果 在0.5~10 mg/L范围内, 哌虫啶浓度与色谱峰面积呈良好线性关系, 相关系数为0.9998, 哌虫啶的最小检出量为1.0 ng。哌虫啶在稻田水、土壤、稻米和小麦粉中的平均添加回收率为78.50%~96.61%, 变异系数为2.54%~7.94%。哌虫啶最低检测浓度为0.05 mg/kg。结论 本方法准确可靠, 操作简便快速, 节约溶剂, 对环境污染小, 符合农药残留分析的要求。

英文摘要:

      Objectives To establish a high performance liquid chromatography (HPLC) method for determination of paichongding (IPP) in paddyfield water, soil, rice and wheat. Methods The paddyfield water was extracted with dichloromethane, while the soil, rice and wheat were extracted with acetonitrile and determined by HPLC with UV detector. Results The results showed a good linearity between the concentration of IPP and the peak areas in the range of 0.5~10 mg/L with r=0.9998. The limit of detection (LOD) was 1.0 ng. The average recoveries were 78.50% to 96.61%, and the relative standard deviations (RSD) ranged from 2.54% to 7.94%. The limit of quantification (LOQ) was 0.05 mg/kg. Conclusion The method is accurate, reliable, simple, rapid, and less organic solvent consumption, and it could meet the requirements of pesticide residue analysis.