超高效液相色谱-串联质谱法测定牛奶中土霉素残留的不确定度评估

Uncertainty Evaluation for the Determination Results of Oxytetracycline in Liquid Milk by UPLC-MS/MS Method

中文关键词:  土霉素  超高效液相色谱-串联质谱法  牛奶  不确定度

英文关键词:oxytetracycline  ultra performance liquid chromatography-tandem mass spectrometry  liquid milk  uncertainty

中文摘要:

      目的 采用超高效液相色谱-串联质谱法对牛奶中土霉素残留的不确定度进行评估。方法 测定牛奶中的土霉素, 建立该方法测定牛奶中土霉素含量不确定度的数学模型, 确定不确定度来源并对其评定。结果 当牛奶中土霉素含量为0.049 μg/g时, 其扩展不确定度为0.0036 μg/g(k=2)。其中, 测量重复性和标准曲线对不确定度的影响最大。结论 采用超高效液相色谱-串联质谱法测定牛奶中土霉素含量应严格控制实验条件, 保证实验具有良好的重复性, 另外还要保证标准曲线具有良好的线性。

英文摘要:

      Objective To establish a mathematical model of uncertainty evaluation for the determination results of oxytetracycline in liquid milk by ultra performance liquid chromatography-tandem mass spectrometry method. Methods The sources of uncertainty of the whole determination procedure were analyzed and the combined uncertainty was finally obtained. Results The expanded uncertainty of 0.0036 μg/g (k=2) with 0.049 μg/g of the content of oxytetracycline was derived. The major sources of uncertainty of measurement included the repeatability of detection and the curve fitting. Conclusion It is necessary to control experimental conditions and the linear of standard curve to make sure the testing results are accurate.