水产品中红霉素残留测定过程中衍生反应条件的优化研究

Study on optimization of derivatization reaction conditions during determination of erythromycin residues

中文关键词:  红霉素  衍生反应  优化

英文关键词:erythromycin  derivatization reaction  optimization

中文摘要:

      对水产品中红霉素残留量测定过程中衍生反应的条件进行探讨和研究。方法 为了使衍生反应达到最佳化, 对影响反应的温度、时间、FMOC-CL的量、反应体系进行了研究。结果 红霉素的最佳衍生条件是反应温度45 ℃、反应时间1 h、50 μg FMOC-CL、反应体系V乙腈:VKH2PO4=2:1。结论 该衍生反应条件优化得比较成功, 适用于所建立的实验方法。

英文摘要:

      Objective To investigate the conditions of derivatization reaction during the process of erythromycin determination in aquatic products. Methods The reaction temperature, reaction time, FMOC-CL quantity and reaction system were studied to optimize the derivatization reaction. Results The results showed that the best derivatization reaction conditions of erythromycin were as follows: the derivatization reaction temperature was 45 ℃, the reaction time was 1 h, and the amount of FMOC-CL was 50 μg under the reaction system of Vacetonitrile:VKH2PO4=2:1. Conclusion The derivatization reaction condition was suitable for the established experiment.