4株红曲霉发酵产生次级代谢产物的分析

Analysis of the secondary metabolites produced by 4 strains of Monascus

中文关键词:  红曲霉  次级代谢产物  γ-氨基丁酸  红曲色素  monacolin K  桔霉素

英文关键词:Monascus  secondary metabolite  γ-aminobutyric acid  monascus pigments  monacolin K  citrinin

中文摘要:

      目的 为研究评价红曲霉产生次级代谢产物能力, 选育优良菌株。方法 通过建立的发酵液中γ-氨基丁酸(GABA)、红曲色素、monacolin K和桔霉素的比色法和高压液相色谱检测方法, 比较了紫色红曲霉(Monascus purpureus)、丛毛红曲霉(Monascus pilosus)、红色红曲霉(Monascus ruber)、橙色红曲霉(Monascus aurantiacus)的液体发酵产生上述次级代谢产物能力。结果 不同红曲霉菌株产生上述4种次级代谢产物的能力是不同的, 4种红曲霉均可产生的红曲色素、monacolin K和桔霉素3种次级代谢产物, 其中M. pilosus J2的monacolin K产量最高(8.1 mg/L)、桔霉素生成量最低(0.09 mg/L); M. purpureus Y4 的红曲色素色价最高, 为380 U/mL; 仅有紫色红曲霉Y4可代谢产生γ-氨基丁酸, 产量约为0.2 g/L。结论 不同红曲霉菌株产生γ-氨基丁酸、红曲色素、monacolin K和桔霉素这4种次级代谢产物能力存在较大差异。

英文摘要:

      Objective To study the ability of secondary metabolites of Monascus, and to breed excellent strains. Methods Detection methods of γ-aminobutyric acid (GABA), monascus pigments, monacolin K and citrinin by colorimetry and HPLC in fermentation were founded, the abilities of secondary metabolites produced by Monascus purpureus, Monascus pilosus, Monascus ruber and Monascus aurantiacus were compared. Results The producing abilities of secondary metabolites among the 4 strains of Monascus were different. The four strains of Monascus could produce monascus pigments, monacolin K and citrinin. The M. pilosus strain J2 had the highest producing ability of monacolin K (8.1 mg/L) and lowest producing ability of citrinin (0.09 mg/L); M. purpureus Y4 had the highest producing ability of monascus pigments producing (380 U/mL), and only M. purpureus Y4 can produceγ-aminobutyric acid (0.2 g/L). Conclusion There are differences in producing γ-aminobutyric acid, monascus pigments, monacolin K and citrinin among different strains