一株多重耐药副溶血性弧菌的耐药分子机制研究

Studies on molecular mechanism of a multi-drug resistant Vibrio parahaemolyticus strain

中文关键词:  副溶血性弧菌  多重耐药性  耐药基因  整合子  喹诺酮耐药决定区

英文关键词:Vibrio parahaemolyticus  multi-drug resistance  drug resistance genes  integrons  quinolone resistance-determining region

中文摘要:

      目的 从养殖半滑舌鳎肠道分离到一株副溶血性弧菌Vp1107, 对该菌株进行耐药性及耐药分子机制研究。方法 通过K-B法进行药敏试验, PCR扩增及测序法对耐药基因及整合子进行检测与分析。结果 Vp1107菌株耐受氨苄西林、阿莫西林、头孢唑啉、四环素、土霉素和氯霉素, 对头孢呋辛钠、链霉素、卡那霉素、萘啶酸、环丙沙星中度敏感, 其多重耐药系数为0.33。菌株携带blaTEM、strA、strB、tetB、tetM、catⅢ、intI1耐药相关基因, 1类整合子不含基因盒, gyrA和parC基因的喹诺酮类耐药决定区未发生点突变。结论 副溶血性弧菌Vp1107耐药程度较为严重, 其耐药性主要由耐药基因编码的耐药性酶类和外排泵作用引起, 提示应加强对副溶血性弧菌耐药性的监控及渔用药物的管理。

英文摘要:

      Objective The antibiotic resistance and the molecular resistance mechanism of a Vibrio parahaemolyticus strain Vp1107 from the gut of the half-smooth tongue sole were studied. Methods The antibiotic resistance was performed by K-B method and the resistance genes and integrons were determined by PCR amplification and DNA sequencing. Results Vp1107 was resistant to ampicillin, amoxycillin, cephazolin, tetracycline, oxytetracycline and chloramphenicol, and intermediate-sensitive to cefuroxime sodium, streptomycin, kanamycin, nalidixic acid and ciprofloxacin. The multi-drug resistance index of this strain was 0.33. blaTEM、strA、strB、tetB、tetM、catⅢ、intI1 genes were present in Vp1107 and class 1 integron was an empty integron without any gene cassettes. There were no mutants within quinolone resistance-determining region of gyrA and parC genes. Conclusion The antibiotic resistance of Vp1107 was relatively severe which was due to inactivated or modified enzymes and efflux pumps, indicating it is necessary to strengthen the monitoring of antibiotic resistance of V. parahaemolyticus and the management of antibiotics’ usage.