应用亲水作用液相色谱法测定食品中过氧化氢

Application of hydrophilic interaction liquid chromatography to determine hydrogen peroxide in food

中文关键词:  过氧化氢  食品  液相色谱法  HILIC色谱柱

英文关键词:hydrogen peroxide  food  high pressure liquid chromatography  HILIC column

中文摘要:

      目的 建立食品中过氧化氢含量的高压液相色谱检测方法。方法 用水提取样品中过氧化氢, 用乙腈做萃取液和流动相, 在1 mL/min流速下, 用Venusil HILIC 色谱柱(250 mm×4.6 mm, 5 μm, 100 ?)不经过衍生化, 直接通过紫外检测器检测食品中的过氧化氢。结果 过氧化氢的检出限为0.6 μg/mL, 定量限为2 μg/mL。对实际样品进行检测时, 平均回收率R≥30%, RSD≤7%。结论 该方法准确、灵敏、精密度高, 可用于食品中过氧化氢的测定。

英文摘要:

      Objective To establish a high pressure liquid chromatography (HPLC) method for determination of the hydrogen peroxide content in food. Methods The food sample was directly extracted with water, and the residual hydrogen perioxide (H2O2) was determined without derivatization via hydrophilic interaction chromatography (HILIC) subsequently. The separation was carried out through a Venusil HILIC column (250 mm×4.6 mm, 5 μm, 100 ?) with acetonitrile as mobile phase at a flow rate of 1 mL/min. Results The detection limit and quantitation limit of H2O2 were 0.6 μg/mL and 2 μg/mL, respectively. The average recovery of food sample was higher than 30% with relative standard deviation (RSD) of lower than 7%. Conclusion This method has a high sensitivity, a good precision and accuracy, which can be applied to determine the H2O2 in food.