衍生化法测定山药中还原型谷胱甘肽(GSH)和总巯基(-SH)含量

Determination of reduced glutathione (GSH) and total thiols (-SH) in Rhizoma Dioscorea by derivatization methods

中文关键词:  山药  还原型谷胱甘肽  4,4-二硫代联吡啶  邻苯二甲醛  衍生化

英文关键词:Rhizoma Dioscore  reduced glutathione  4,4-dithiodipyridine  O-phthaldehyde  derivatization

中文摘要:

      目的 用衍生化法测定山药中还原型谷胱甘肽(GSH)和总巯基(-SH)的含量。方法 利用4,4?-二硫代联吡啶(4-DPS)和邻苯二甲醛(OPA)为衍生化试剂,采用细胞破壁和衍生化为一体的样品前处理方法, 分别用UV-vis分光光度法和荧光光谱法测定了山药鲜品中总巯基(-SH)、GSH的含量。结果 山药中含较丰富的总巯基和GSH, 含量分别达0.4556 ?mol/g和0.1792 ?mol/g。UV-vis分光光度法和荧光光谱法测定方法的检测限分别为1.0458 ?mol/L和0.5666 ?mol/L, 回收率分别为98.73%和98.40%。结论 本研究对山药中巯基化合物的含量、测定方法学和样品前处理方法提供了重要的基础数据和参考依据。

英文摘要:

      Objective To detect reduced glutathione (GSH) and total thiols (-SH) in Rhizoma Dioscorea by derivatization methods. Methods Total thiols and reduced glutathione (GSH) in Rhizoma Dioscorea were determined by UV-vis spectrophotometry and fluorophotometry using 4,4?-dithiodipyridine (4-DPS) and O-phthaldehyde (OPA) as derivatization agents. Samples were prepared by simultaneous derivatization during cell rupture, Results 0.4556 μmol/g of total thiols and 0.1792 μmol/g of GSH were detected in the samples . The detection limits for UV-vis spectrophotometry and fluorophotometry were 1.0458 μmol/L and 0.5666 μmol/L, and recoveries were 98.73% and 98.40%, respectively. Conclusion This study provides useful data and references for the content of thiol compounds in Rhizoma Dioscore determination methodology and pretreatment methods for plant sample analysis.