人乳铁蛋白转基因和非转基因山羊奶中 蛋白质含量与必需氨基酸总量的检测与分析

Detection and analysis on protein content and EAA amounts in the human lactoferrin transgenic and non-GMO goats’ milk

中文关键词:  人乳铁蛋白  转基因山羊奶  非转基因山羊奶  蛋白质含量  必需氨基酸总量  分析

英文关键词:human lactoferrin  transgenic goats’ milk  non-GMO goats’ milk  protein content  total amount of essential amino acids  analysis

中文摘要:

      目的 测定人乳铁蛋白转基因山羊奶及非转基因山羊奶中蛋白质含量和7种必需氨基酸总量, 考察两种羊奶的上述两种成分是否存在显著差异, 为人乳铁蛋白转基因山羊奶的实质等同性评价提供依据。方法 采用凯氏定氮法测定两种山羊泌乳30、60、90 d采集的羊奶中各90个样本的蛋白质含量; 采用OPA-FMOC柱前衍生高效液相色谱法测定山羊奶中的7种必需氨基酸总量, 用SPSS软件对测定结果进行统计学处理。结果 泌乳30、60、90 d采集的样本中, 转基因山羊奶的平均蛋白质含量分别为43.4、36.0、35.6 g/L, 7种必需氨基酸的平均总量分别为17.7、14.9、14.8 g/L; 非转基因山羊奶的平均蛋白质含量分别为52.2、49.1、41.3 g/L, 7种必需氨基酸的平均总量分别为 21.4、21.4、18.1 g/L。结论 在3个时间段采集的样本中, 转基因与非转基因山羊奶中的蛋白质含量均存在显著性差异(P≤0.05)。除30 d天采集的样本外, 60 d及90 d采集的样本中, 两种羊奶的7种必需氨基酸总量均存在显著性差异(P≤0.05)。

英文摘要:

      Objective To provide the reference for substantial equivalence evaluation on the quality of human lactoferrin transgenic goats’ milk, the protein content and total amount of 7 kinds’ essential amino acid (EAA) in human lactoferrin transgenic and non-GMO goats’ milk were determined, and the difference in the above two components between the two kinds of goats’ milk was investigated. Methods The Kjeldahl Method was used to determine the protein content of 90 samples from two kinds of goats’ milk, which were collected in the 30th, 60th and 90th lactating day. Total amount of 7 kinds’ EAA was tested by HPLC with pre-column OPA&FMOC derivatization. All the data were analyzed with SPSS software. Results For the samples from the 30th, 60th and 90th lactating day, the average value of protein content in transgenic goats’ milk was 43.4, 36.0, 35.6 g/L respectively, while that in non-GMO goats’ milk was 52.2, 49.1, 41.3 g/L. The average value of total amount of 7 kinds’ EAA in transgenic goats’ milk was 17.7, 14.9, 14.8 g/L respectively, while that in non-GMO goats’ milk was 21.4, 21.4, 18.1 g/L. Conclusion For the samples from the 30 th, 60 th and 90 th lactating day, there was a significant difference in protein content between the two kinds of goats’ milk (P≤0.05). Except for the samples from the 30th lactating day, there was a significant difference in total amount of 7 kinds’ EAA between the two kinds of goats’ milk (P≤0.05).