分光光度法测定大豆卵磷脂中磷脂酰胆碱含量

Determination of phosphatidylcholine in soybean lecithin by spectrophotometry

中文关键词:  卵磷脂  磷脂酰胆碱  碱水解  分光光度法  雷氏盐

英文关键词:lecithin  phosphatidylcholine  alkaline hydrolysis  spectrophotometry  Reinecke’s salt

中文摘要:

      目的 建立一种简单、准确的卵磷脂测定方法。方法 采用KOH溶液将磷脂酰胆碱中的胆碱游离, 加雷氏盐反应后, 在波长525 nm处, 通过分光光度法测定胆碱的量。结果 该方法在0.075～0.225 mmol 范围内线性关系良好, 线性方程为Y = 3.6457X+0.026, R2 = 0.9987, 方法的回收率在95%～103%之间, 相对标准偏差小于1.0%, 待测液在2.5 h之内稳定, 能够满足检测要求。结论 本方法具有操作简单、准确性高、稳定性好、抗干扰性强的优点, 可以用于卵磷脂产品中磷脂酰胆碱含量的测定。

英文摘要:

      Objective To establish a simple and accurate method for determination of phosphatidylcholine. Methods Free choline was obtained from phosphatidylcholine by alkaline hydrolysis and was determined by spectrohpotometry under the wavelength of 525 nm after reaction with Reinecke’s salt. Results The method showed a good linearity over the range of 0.075m mol～0.225m mol,and the linear equation was Y = 3.6457X+0.026 with R2=0.9987. The recoveries was in range of 95%～103%, and the relative standard deviations (RSDs) were lower than 1%. The liquid was stable in 2.5h, which can meet the testing requirements. Conclusion This method is simple, accurate, stable, and anti-interference, and it can be used for the determination of phosphatidylcholine from lecithin products.