MALDI-TOF-MS方法检测、鉴定副溶血性弧菌

Detection and identification of Vibrio parahaemolyticus by matrix assisted la-ser desorption/ionization time of flight mass spectrometry

中文关键词:  MALDI-TOF-MS  副溶血性弧菌  检测  鉴定

英文关键词:matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF- MS)  Vibrio parahaemolyticus  detection  identification

中文摘要:

      目的 建立快速检测和鉴定副溶血性弧菌的基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF-MS)分析方法。方法 采用副溶血性弧菌标准菌株, 在不同的培养基或不同的培养时间下进行MALDI-TOF-MS检测, 同时对长时间冷冻保存的菌株进行检测, 以验证该方法的稳定性和重复性。对不同来源的菌株进行检测, 以确认方法的准确性。结果 用PW、TCBS琼脂和弧菌显色培养基等3种培养基, 分别培养18、42、72 h时, 对该菌的蛋白质谱图影响很小；保存2年的菌株, 其MALDI-TOF-MS鉴定分值保持稳定；对来自不同国家和地区的菌株都可以得到同样准确的结果。结论 MALDI-TOF-MS方法可以快速、准确地鉴定副溶血性弧菌。因其具有很好的稳定性和重复性, 可用于副溶血性弧菌的日常检测。

英文摘要:

      Objective To establish a rapid method for the detection and identification of Vibrio para?hae?moly?ticus by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Methods In order to verify the stability and repeatability, the reference strain V. parahaemolyticus cultured in different medium or different time and the isolates which had been cryopreserved for a long time were de-tected by MALDI-TOF-MS. The strains from different sources had also been tested to confirm the accuracy of this method. Results Different medium such as PW, TCBS and Vibrio colored medium and different culturing time interval of 18, 42 or 72 h had little impact for the mass spectrometry of the bacteria. There were no changes for the identification scores of the bacteria which had been preserved for two years using MALDI- TOF-MS. The strains from different countries and regions get the same accurate results. Con-clusion MALDI- TOF-MS is a fast and accurate method for the identification of V. parahaemolyticus by MALDI-TOF-MS, and it can be used for routine detection because of its good stability and repeatability.