绞股蓝皂苷成分的分离与鉴定

Isolation and characterization of gypenosides from Gynostemma pentaphyllum

中文关键词:  绞股蓝  达玛烷类皂苷  gypenoside XLVI  gypenoside LVI

英文关键词:Gynostemma pentaphyllum  dammarane-type saponin  gypenoside XLVI  gypenoside LVI

中文摘要:

      目的 提取、分离、鉴定绞股蓝皂苷主要成分。方法 利用硅胶柱色谱及反相半制备柱色谱等分离手段, 分离、纯化绞股蓝皂苷成分, 并用核磁共振波谱(1H-NMR、13C-NMR)、液相色谱离子阱飞行时间质谱(LCMS-IT-TOF)等方法鉴定化合物结构, 并对相关成分的抑癌作用进行了研究。结果 绞股蓝总提物的主要成分经分离得到2种达玛烷类皂苷, 分别为绞股蓝皂苷gypenoside XLVI和gypenoside LVI, 而且它们具有一定的抑制癌细胞增殖作用。结论 利用硅胶柱色谱和反相半制备色谱方法从绞股蓝总提物中分离得到主要的皂苷成分, 为进一步研究其抑癌有效成分提供了参考。

英文摘要:

      Objective To establish a method for the isolation and characterization of gypenosides from Gynostemma pentaphyllum. Methods Gypenosides were isolated with chromatography using silica gel and reversed semi-preparative HPLC and identified with 1H-nuclear magnetic resonance spectrum, 13C- nuclear magnetic resonance spectrum and liquid chromatography-ion trap time of flight tandem mass spectrometry. Results　The two constituents isolated from Gynostemma pentaphyllum were identified as dammarane-type saponins, gypenoside XLVI and gypenoside LVI, respectively. Conclusion Ggypenosides can be isolated effectively by silica gel chromatography and reversed semi-preparative HPLC.