气相色谱-质谱法测定鸡肉中二氯二甲吡啶酚残留量

Determination of dichloro dimethyl pyridine phenol residues in chicken tissues by gas chromatography-mass spectrometry

中文关键词:  二氯二甲吡啶酚  气相色谱-质谱法  鸡肉  鸡肝  检测

英文关键词:dichloro dimethyl pyridine phenol  gas chromatography-mass spectrometry  chicken tissues  chicken liver  determination

中文摘要:

      目的 建立鸡肉中二氯二甲吡啶酚残留量的气相色谱-质谱分析方法。方法 样品经甲醇提取, 中性氧化铝柱净化, 在碱性条件下以吡啶催化, 丙酸酐衍生后, 用气相色谱-质谱仪测定。结果 二氯二甲吡啶酚在20~240 ?g/L浓度范围内与响应值呈良好的线性关系, 线性回归方程为Y=1.132X?2.553, 相关系数大于0.998。该方法的平均回收率为78.7%~85.1%, 相对标准偏差(RSD)为7.8%~12.6%, 检出限(LOD)为: 鸡肉5.0 μg/kg, 鸡肝10.0 μg/kg。结论 该方法选择性好, 适合鸡肉中二氯二甲吡啶酚残留量的测定。

英文摘要:

      Objective To establish a method for the determination of dichloro dimethyl pyridine phenol residues in chicken tissues by gas chromatography-mass spectrometry (GC-MS). Methods Dichloro dimethyl pyridine phenol was extracted with methanol and cleaned-up though Al2O3 column and derived with propionic anhydride under alkaline conditions using pyridine as catalyst. Quantification was achieved by GC-MS. Results The recoveries of dichloro dimethyl pyridine phenol in chicken tissues were in the range of 78.7%~ 85.1% with the relative standard deviation (RSD) of 7.8%~12.6%. The limit of detection (LOD) was 5.0 μg/kg for chicken and 10.0 μg/kg for chicken livers. Conclusion The method has good selectivity and it is suitable for the determination of dichloro dimethyl pyridine phenol residues in chicken tissues.