| 张舒亚,李富威,于翠,宋青,张坤.环介导等温扩增法检测食品过敏原大豆成分[J].食品安全质量检测学报,2013,4(2):451-456 |
| 环介导等温扩增法检测食品过敏原大豆成分 |
| Detection of allergen soybean by loop-mediated |
| 投稿时间：2012-11-26 修订日期：2013-01-19 |
| DOI： |
| 中文关键词: 食品过敏原 大豆 LAMP 检测 |
| 英文关键词:food allergen soybean loop-mediated isothermal amplification detection |
| 基金项目:上海科委技术标准专项(12DZ0503103)、上海出入境检验检疫局科研项目(HK006-2013) |
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| 中文摘要: |
| 目的 建立食品过敏原大豆成分的环介导等温扩增(LAMP)分析方法。方法 根据大豆的内源管家基因Lectin基因设计6条过敏原大豆的特异性引物(两条内引物, 两条外引物和两条环引物), 建立LAMP检测方法。结果 环介导等温扩增法在63 ℃条件下, 40 min内可检出过敏原大豆成分, 该方法能够有效对大豆成分进行快速检测, 具有较强的特异性, 灵敏度可达0.01%(w/w)大豆粉。结论 该方法特异性强, 灵敏度高, 可以快速、准确检测食品中过敏原大豆成分。 |
| 英文摘要: |
| Objective To establish a detection method of allergen soybean by loop-mediated isothermal amplification assay (LAMP). Methods The method of LAMP was established for the detection of soybean in food. Results The soybean could be rapidly and specifically detected by LAMP method within 40 min at constant temperature of 63 ℃. The method was specific for soybean, and the limit of detection was determined as 0.01% (w/w) soybean powder. Conclusion The established LAMP method is effective, specific and sensitive for the detection of soybean, and it is suitable for the detection of soybean in food. |
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