液质联用法测定桃中三种杀菌剂的含量分布

Distribution of three fungicides in peaches determined by liquid chromatography–tandem mass spectrometry

中文关键词:  多菌灵  甲基硫菌灵  敌菌灵  液质联用  桃  含量分布

英文关键词:carbendazim  thiophanate-methyl  anilazine  liquid chromatography–tandem mass spectrometry  peaches  distribution

中文摘要:

      目的 建立桃皮和肉中多菌灵、甲基硫菌灵和敌菌灵三种杀菌剂的液质联用(LC-MS/MS)测定方法, 研究其在桃中的含量分布。方法 桃皮和肉经分离后, 分别用乙腈均质提取、PSA固相分散净化、C18高压液相色谱柱分离、MS/MS测定; 分别计算皮和肉中三种杀菌剂的含量, 研究三种杀菌剂在两者间的分布状况。结果 方法检测限分别为0.1、0.2、10 μg/kg; 敌菌灵在样品中未检出, 多菌灵和甲基硫菌灵在皮和肉中呈不同的含量分布特性。结论 该方法灵敏度高、准确、操作简单, 可用于桃中三种杀菌剂的含量分布研究。

英文摘要:

      Objective To establish a method for the determination of three kinds of fungicides (carbendazim, thiophanate-methyl and anilazine) in the peel and pulp of peaches by liquid chromatography–tandem mass spectrometry (LC-MS/MS) and study their distributions in peaches. Methods The peel and pulp separated from peaches were extracted with acetonitrile by homogenization, purified by solid-phase dispersion ex-traction with PSA, separated by C18 HPLC column and measured by MS/MS, respectively. The concentrations of three kinds of fungicides in peel and pulp were calculated respectively. The results were used to study the distribution levels in peaches. Results The LODs were 0.1, 0.2 and 10 μg/kg for carbendazim, thiophanate-methyl and anilazine, respectively. Anilazine was not detected in samples. Different distribution results were found for carbendazim and thiophanate-methyl in peel and pulp. Conclusion The method is sensitive, accurate and easy to operate. It is suitable for the distribution study of these fungicides in peaches.