阻抗生物传感器连续叠加法检测毒死蜱研究

Additive assay method for detection of chlorpyrifos using impedance biosensor

中文关键词:  毒死蜱  阻抗生物传感器  叉指微电极  农药残留  叠加法

英文关键词:chlorpyrifos  impedance biosensor  interdigitated microelectrode  pesticide residue  additive assay

中文摘要:

      目的 研究微电极阻抗生物传感器快速检测农药以及回归模型建立。方法 用蛋白A修饰叉指微电极, 把5个不同浓度的毒死蜱样品依次滴加到已经制备好的阻抗生物传感器上进行检测, 分析6个特定频率下毒死蜱浓度与阻抗信号的相关性。结果 在10 Hz频率下, 毒死蜱浓度在0.01 μg/mL~100 μg/mL范围内, 建立的回归模型方程为: Y=136.4Ln(X)+2075, 相关系数R2为0.961。结论 该方法具有操作简单、检测速度快等优点, 可以适用于其他生物传感器研究。

英文摘要:

      Objective To develop an impedance biosensor based on interdigitated microelectrode for rapid detection of pesticide residue and establish its regression model. Methods The microelectrode was modified by protein A. And five sample solutions with different concentrations of chlorpyrifos were added to the pre-pared impedance biosensor for analysis. Then the relationships between chlorpyrifos concentration and its impedance value under six selected frequencies were analyzed. Results The regression equation was Y= 136.4Ln(X)+2075 (R2 =0.961), under 10 Hz frequency and chlorpyrifos concentration between 0.01~100 μg/mL. Conclusion The method is simple and rapid, and can be used in field of other biosensor research.