加速溶剂萃取-气相色谱质谱法测定土壤中多环芳烃的含量

Determination of polycyclic aromatic hydrocarbons residues in soil by GC/MS equipped with accelerated solvent extraction

中文关键词:  土壤  多环芳烃  加速溶剂萃取法  固相萃取  气相色谱质谱法

英文关键词:soil  polycyclic aromatic hydrocarbons  accelerated solvent extraction  solid phase extraction  gas chromatography-mass spectrometry

中文摘要:

      目的 建立土壤中7种多环芳烃(PAHs)的气相色谱质谱测定方法。方法 采用加速溶剂萃取法(ASE), 以二氯甲烷和丙酮(1∶1, v/v)为溶剂, 萃取液通过弗罗里硅土固相萃取柱净化后, 用GC/MS检测, 检测模式为选择离子监测(SIM)模式, 利用保留时间和特征离子进行定性, 外标法定量。结果 7种多环芳烃线性关系良好, 相关系数均大于0.995, 加标回收率均在87.2%~129%范围内, 最低检出限在0.096~1.48 μg/kg之间。结论 该方法操作方便、灵敏度高、重现性好, 可用于土壤中痕量多环芳烃的测定。

英文摘要:

      Objective To establish a method for the determination of seven polycyclic aromatic hydrocarbons (PAHs) residues in soil by GC/MS. Methods The soil samples were extracted with accelerated solvent extraction (ASE) using a mixture of dichloromethane and acetone(1: 1, v/v), purified with solid phase extraction (SPE) and analyzed by gas chromatography -mass spectrometry (GC/MS) under selected ion mode (SIM). Reten-tion time and characteristics ions were used for qualification and external standard method was used for quantitation. Results All of the seven PAHs standards showed a good linearity (r >0.995). The recoveries were between 87.2% and 129% and the detection limits were 0.096~1.48 μg/kg. Conclusion This method is simple, sensitive and reproductive, and it is suitable for the determination of seven PAHs residues in soil.