HPLC-DAD法同时测定高脂肪食品中黄色2G、奶油黄、柑橘红2和苏丹红Ⅰ~Ⅳ

HPLC-DAD simultaneous determination of yellow 2G, butter yellow, citrus red 2 and sudan red I~IV in high fat foods

中文关键词:  凝胶渗透色谱净化  高压液相色谱法  黄色2G  奶油黄  柑橘红2  苏丹红  高脂肪食品

英文关键词:gel permeation chromatography purification  high pressure liquid chromatography  yellow 2G  butter yellow  citrus red 2  sudan red  high fat food

中文摘要:

      目的 建立同时测定高脂肪食品中黄色2G、奶油黄、柑橘红2和苏丹红Ⅰ~Ⅳ的HPLC-DAD法。方法 样品经环已烷/乙酸乙酯(1∶1, v/v) 萃取, Bio-Beads SX3 凝胶层析柱净化去除油脂、天然色素等大分子干扰物质。采用C18色谱柱, 以甲醇和酸性水溶液(pH 4.0)为流动相梯度洗脱, 二极管阵列检测器多波长同时检测这七种色素。结果 在1.0~10.0 mg/L范围内, 峰面积与进样量呈良好的线性关系, 线性相关系数大于0.999, 回收率在81.0%~98.2%, 相对标准偏差在0.89%~5.21%。黄色2G、奶油黄、柑橘红2的检出限均达到1.0 mg/kg, 苏丹红Ⅰ~Ⅳ色素的检出限均达到2.0 mg/kg。结论 该方法简便、快捷、灵敏、准确、重现性好, 能满足相关色素同时检测的需要。

英文摘要:

      Objective To establish an HPLC-DAD method for simultaneous determination of yellow 2G, butter yellow, citrus red 2 and sudan red I~IV. Methods Samples were extracted directly with a mixture of cyclohexane-athylacetate (1∶1, v/v ) and purified by a Bio-beads SX3 gel column, to remove interfering high molecular fatty substances and natural pigments in food samples. HPLC was carried out on a C18 column with MeOH H2O (pH 4.0) as mobile phase in the gradient elution. Multiwavelength diode array detection (DAD) was applied in the determination of the 7 kinds of pigments. Results Good linear relationships in the range of 1.0~10 mg/L of 7 compounds were obtained with correlation coefficients (r) all above 0.999. The RSDs were in the range of 0.89%~5.21%, and average recoveries were 81.0%~98.2%. The detection limits were 1.0 mg/kg for yellow 2G, butter yellow, citrus red 2 and 2.0 mg/kg for sudan red I~IV, respectively. Conclusion The established method is simple, rapid, sensitive, accurate and repeatable, and can be used for the simultaneous determination of relevant pigments.