基于电沉积壳聚糖-碳酸钙-纳米金的有机磷农药生物传感器

Electrodeposited chitosan-calcium carbonate-gold nanoparticles composite film based biosensor for organophosphorous pesticide

中文关键词:  乙酰胆碱酯酶  纳米金  碳酸钙  乐果

英文关键词:acetylcholinesterase  gold nanoparticles  calcium carbonate  dimethoate

中文摘要:

      目的 制备基于壳聚糖-碳酸钙-纳米金复合膜固定乙酰胆碱酯酶的新型电化学传感器。方法 一步电沉积壳聚糖、碳酸钙和纳米金修饰到玻碳电极, 由于电沉积过程有气泡产生, 使沉积到电极上的碳酸钙微粒形成了三维多孔的结构, 利于酶比较分散的固定在电极表面, 提高检测效率; 同时纳米金的引入不仅增大了电极比表面积, 而且促进电子的快速传递, 放大了检测信号。结果 将制备的生物传感器用来检测有机磷农药乐果, 检测限为2.5 pg/mL, 检测时间只需要7 min。结论 该生物传感器检测法实现了对有机磷农药快速、超灵敏的检测。

英文摘要:

      Objective To develop a novel biosensor based on chitosan-calcium carbonate-gold nanoparticles composite film immobilizing acetylcholinesterase Methods The chitosan, calcium carbonate and gold nanoparticles were one-step electrodeposited onto a glassy carbon electrode. Due to the bubble generated in the electrodeposit process, the generated micro-CaCO3-based matrix showed a three-dimensional porous structure, providing a favorable and biocompatible micro-environment to dispersedly immobilize enzyme onto it and improve the detection effect. With the introduction of gold nanoparticles, the signal was amplified and the transmission of electrons was accelerated. Results The prepared biosensor showed a favorable electrochemical behavior with a detection limit of 2.5 pg/mL and detection time of 7 min for dimethoate. Conclusion The established method is ultrasensitive and rapid, and can be used to detect organophosphorous pesticide.