不同水产品中有色和无色孔雀石绿的降解动力学研究

Studies on degradation kinetics of malachite green and leucomalachite green in different aquatic products

中文关键词:  有色孔雀石绿  无色孔雀石绿  水产品  降解动力学

英文关键词:malachite green  leucomalachite green  aquatic products  degradation kinetics

中文摘要:

      目的 研究不同水产品基质中有色和无色孔雀石绿的降解规律。方法 样品经提取净化后, 用HPLC进行检测, 结果用SPSS软件、降解动力学软件进行分析。结果 三种基质中有色和无色孔雀石绿降解反应均符合准一级反应动力学。在10、50、100 μg/kg三个添加水平下, 三种基质中有色孔雀石绿平均降解率分别为57.9%、48.0%、23.0%, 无色孔雀石绿平均降解率分别为46.6%、43.4%、19.8%。添加浓度为50 μg/kg的鲤鱼基质, 采用自然解冻时有色和无色孔雀石绿降解速率常数分别为K(50 μg/kg)=0.00338 d?1, K(50 μg/kg)=0.00268 d-1, 采用微波解冻方式时有色和无色孔雀石绿降解速率常数分别为K(50 μg/kg)=0.00359 d?1, K(50 μg/kg)=0.00321 d?1。实际样品中无色孔雀石绿降解速率常数K(111.2 μg/kg无色, 鲤鱼)=0.000614 d?1, K(50 μg/kg无色, 鲤鱼)=0.00125 d?1。结论 有色孔雀石绿较无色孔雀石绿降解快, 低浓度比高浓度降解快; 两种解冻方式对有色和无色孔雀石绿降解影响较小; 实际样品中无色孔雀石绿降解速率常数均比空白基质中添加无色孔雀石绿降解慢。

英文摘要:

      Objective To study degradation kinetics of malachite green and leucomalachite green in different aquatic products. Methods After extracted and purified, samples were detected by high pressure liquid chromatography, and the results were analyzed with SPSS software and degradation kinetics software. Results Degradation of malachite green and leucomalachite green in different aquatic products’ matrix was fit to pseudo-first order kinetics equations．The degradation rates of malachite green were 57.9%, 48.0%, 23.0%, and the degradation rates of leucomalachite green were 46.6%, 43.4%, 19.8% in three aquatic products’ matrixes, respectively. For carp matrix added concentration of 50 μg/kg, malachite green and leucomalachite green degradation rate constants were K (50 μg/kg)=0.00338 d?1, K (50 μg/kg)=0.00268 d?1 in natural thawing.and K (50 μg/kg) = 0.00359 d?1, K (50 μg/kg)=0.00321 d?1 in microwave thawing, respectively. The leucomalachite green degradation rate constants in practical samples were K(111.2 μg/kg carp )=0.000614 d?1, and K(50 μg/kg carp )=0.00125 d?1. Conclusion Degradation rates of malachite green was faster than that of leucomalachite green. Compared with higher concentration, the degradation in low concentration was faster. Two kinds of ways of thawing had no effects on the degradation of malachite green and leucomalachite green. The leucomalachite green degradation rate in practical samples was slower than that in the blank matrix added of leucomalachite.