贝类毒素检测中两种筛选方法的比较研究

Comparison of two screening methods in detection of shellfish poisons

中文关键词:  贝类毒素  小鼠生物法  酶联免疫吸附法  快速筛选  检测

英文关键词:shellfish poison  mouse bioassay  ELISA  rapid screening  detection

中文摘要:

      目的 采用传统小鼠生物法(MBA)和酶联免疫吸附法(ELISA)对贝类样品中四类毒素进行检测, 为不同要求下建立或选择准确的贝类毒素检测快速筛选方法提供参考。方法 分别采用MBA和ELISA检测腹泻性贝毒(DSP)和麻痹性贝毒 (PSP), 并采用ELISA检测记忆缺损性贝毒(ASP) 和神经性贝毒(NSP)。结果 对2009~2011年8种67份贝类样品进行检测, 结果表明: 两种测试方法在实际应用中对DSP、PSP检测结果不存在差异, 检测结果有很好的吻合性。使用ELISA法对自制ASP、NSP模拟阳性样品进行检测, 均测得ASP、NSP, 检测结果满意。结论 两种筛选方法在贝类毒素检测中均有其应用空间。实验室可根据不同情况选择合适的检测方法。

英文摘要:

      Objective To determine four kinds of toxins in shellfish by using the methods of mouse bioassay(MBA) and enzyme-linked immunosorbent assay(ELISA) and provide references for the rapid detection of toxins in shellfish. Methods Diarrhetic shellfish poison and paralytic shellfish poison were detected by MBA and ELISA, while amnesic shellfish poison and neurotoxic shellfish poison were detected by ELISA. Results The experiment was conducted using a total of 8 kinds of 67 samples and the results showed that there were no differences between the detection results of diarrhetic shellfish poison and paralytic shellfish poison using the two methods. The result obtained by MBA was in good accordance with that of ELISA. Conclusion These two kinds of rapid screening methods in the detection of shellfish poison has its own application scope. The laboratory can choose a suitable detection method based on different situations.