高效液相色谱法检测粮谷及其制品中六种A, B型单端孢霉烯族真菌毒素

Determination of six type A and type B trichothecenes by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  A,B型单端孢霉烯族真菌毒素  高效液相色谱?串联质谱  粮谷及其制品

英文关键词:type a and type b trichothecenes  high performance liquid chromatography-tandem mass spectrometry  grain and grain-products

中文摘要:

      目的 建立粮谷及其制品中六种A,B型单端孢霉烯族真菌毒素的高效液相色谱?串联质谱分析方法。方法 样品经乙腈?水溶液(84︰16, V/V)提取, MycoSep?226或MycoSep?227多功能柱净化, Waters Atlantis dC18色谱柱分离, 串联四极杆质谱多反应离子监测模式检测。结果 6种真菌毒素在各自的线性范围内线性关系良好, 相关系数均不低于0.996, 定量下限为0.1~20 μg/kg, 低、中、高3个加标水平下平均回收率为60.1%~123%, 相对标准偏差为7.5%~19.8% 。结论 该方法具有前处理简单、净化效果好、灵敏度高的优点, 适用于复杂基质样品中A,B型单端孢霉烯族真菌毒素残留的确证和定量检测。

英文摘要:

      Objective To establish a method for the simultaneous determination of six type A and type B trichothecenes by high performance liquid chromatography-tandem mass spectrometry. Method The homogenized sample was extracted by 84% acetonitrile-water solution (V/V) and further purified by MycoSep?226 or MycoSep?227. The target compounds were separated by waters Atlantis dC18column and detected by MS/MS system with eletrospray ionization (ESI) under multiple reaction monitoring (MRM) mode. Results It was shown that the high correlation coefficients (r≥0.996) of six mycotoxins were obtained within their respective linear ranges. The LOQ for the six compounds ranged from 0.1~20 μg/kg. The mean recoveries ranged from 60.1%~123% with the relative standard deviations of 7.5%~19.8%. Conclusion This method was suitable for the identification and quantification of multi-component mycotoxin contaminants in complex matrixes with advantages of simple pretreatment, good purification and high sensitivity.