固相萃取-高效液相色谱-荧光检测法联合测定食品中苯并(a)芘

Determination of benzopyrene in food by solid phase extraction-high performance liquid chromatography with fluorescent detector

中文关键词:  苯并(a)芘  食品  高效液相色谱?荧光法  固相萃取

英文关键词:benzopyrene  foodstuffs  HPLC-FLD  solid phase extraction

中文摘要:

      目的 采用固相萃取?高效液相色谱?荧光法(HPLC-FLD)联合测定食品中苯并(a)芘的含量。方法 根据不同样品基质对样品提取条件进行了优化, 采用固相萃取柱净化, XbridgeTMC18色谱柱分离, 外标法定量。结果 苯并(a)芘在0~100 ng/mL浓度范围内线性关系良好, 相关系数r=0.9994。本方法的测定低限(S/N≥10): 茶油、罗非鱼和猪肉为1.0 μg/kg, 大米、包菜、茶叶、饮用水和橙汁为0.5 μg/kg; 添加浓度为0.5 μg/kg ~100 μg/kg时, 回收率范围为77.3% ~93.2%, 变异系数(n≥6)为0.65% ~7.18%。结论 该方法前处理简单, 分析时间短, 具有良好的灵敏度和准确性, 适用于各类食品中苯并(a)芘的测定。

英文摘要:

      Objective A method of solid phase extraction-high performance liquid chromatography with fluorescent detector(HPLC-FLD)was developed for determination of benzopyrene (BaP) in foodstuffs. Method The analytical procedure includes optimization of extraction conditions, purification using a SPE column and separation by XbridgeTMC18 Chromatographic column, followed by a quantitative external standard method. Results The method showed a good linearity over the range of 0~100 ng/mL for benzopyrene (BaP) with r=0.9994. The detection limits (S/N≥10) were 1.0 μg/kg for tea seed oil, tilapia, pork and 0.5 μg/kg for rice, cabbage, tea, water and orange juice. The recoveries were 77.3%~93.2% at spiked levels of 0.5 μg/kg~100 μg/kg and the relative standard deviations were 0.65%~7.18% (n≥6). Conclusion This method has the advantages of easy to operate, fast to perform, with high sensitivity and accuracy, and it was suitable for the detection of benzopyrene in foodstuffs.