| 耿志明,杨丹,张平平,张旭,杨学明,马鸿翔.三氟甲烷磺酸水解-HPLC法测定小麦中结合态脱氧雪腐镰刀菌烯醇[J].食品安全质量检测学报,2012,3(1):27-32 |
| 三氟甲烷磺酸水解-HPLC法测定小麦中结合态脱氧雪腐镰刀菌烯醇 |
| Determination of conjugated deoxynivalenol content in wheat by HPLC after hydrolysis with trifluoromethanesulfonic acid |
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| DOI： |
| 中文关键词: 脱氧雪腐镰刀菌烯醇 结合态脱氧雪腐镰刀菌烯醇 三氟甲烷磺酸 高效液相色谱法 |
| 英文关键词:deoxynivalenol conjugated deoxynivalenol trifluoromethanesulfonic acid high performance liquid chromatograph |
| 基金项目:国家科技部国际合作项目(2009DFA32020)和小麦产业技术体系项目(CARS-03) |
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| 中文摘要: |
| 目的 建立三氟甲烷磺酸水解?高效液相色谱(HPLC)测定小麦中结合态脱氧雪腐镰刀菌烯醇(DON)含量的分析方法。方法 通过3因素4水平正交试验, 确立三氟甲烷磺酸水解小麦中结合态DON的最佳条件。结果 水解温度60 ℃, 酸浓度1.0 mol/L, 时间60 min为最佳检测条件。 检测了7个小麦样品中的结合态DON含量, 在DON污染的小麦样品中均检出有结合态DON, 其含量与游离态DON之比为0.20~0.36。结论 所建方法具有良好的可靠性和实用性。 |
| 英文摘要: |
| Objective To establish the method for determination of conjugated deoxynivalenol (DON) content in wheat by high performance liquid chromatograph (HPLC) after hydrolysis with trifluoromethanesulfonic acid (TFMSA). Methods The optimum conditions for hydrolysis of conjugated DON with TFMSA were set up by an orthogonal design, in which temperature was 60 ℃, TFMSA was 1.0 mol/L and duration of hydrolysis was 60 min, respectively. Results Conjugated DON contents in 7 wheat samples were analyzed, and 5 samples were found containing conjugated DON with their ratios to free DON varying from 0.20 to 0.36. Conclusion The method was of good reliability and practicality. |
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