人为添加非蛋白质氮乳与乳制品中蛋白质含量测定方法的研究

Research on the methods of protein determination after added non-protein in milk and dairy products

中文关键词:  乳及乳制品  蛋白质  双缩脲比色法  非蛋白氮  

英文关键词:milk and dairy products  protein  Biuret spectrophotometry methord  non-protein nitrogen  

中文摘要:

      本研究分别用GB/T5009.5《食品中蛋白质的测定》和NY/T1678《乳与乳制品中蛋白质的测定——双缩脲比色法》,测定了添加非蛋白氮物质的乳及乳制品中的蛋白质含量。结果表明GB/T5009.5方法不能区分样品中的蛋白氮与非蛋白氮;采用NY/T1678-2008方法可以较好的实现蛋白氮和非蛋白氮物质的分离,有效地排除三聚氰胺、尿素、甘氨酸和水解蛋白等多种非蛋白氮对乳及乳制品中蛋白质含量检测的影响,该方法前处理方法快速、简单,检测结果准确。 更多还原

英文摘要:

      The contents of protein were determined using both "GB/T 5009.5 determination of protein in foods" method and "NY/T 1678 determination of protein in milk and dairy products with Biuret spectrophotometry" method,after adding non-protein nitrogen in milk and dairy products.It is shown from the results that GB/T 5009.5 method can not distinguish between protein nitrogen and non-protein nitrogen.Compared with the GB/T 5009.5 method,the NY/T 1678 method can determined the contents of protein accurately after adding melamine and Urea or Proteinum hydrolysatum in milk and dairy products.The method is accurate,simple and rapid.